Simultaneous estimation of contraceptive steroids from electrospun fibers by RP-HPLC method: development, validation and application

Nanofibers-based drug delivery systems are promising alternative to formulate transdermal drug formulations, which are suitable for sustained release of dual drugs in combined drug systems. The parameters such as content of active ingredients and physical/chemical stability of a drug are essential t...

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Bibliographic Details
Published in:Journal of the Iranian Chemical Society 2021-12, Vol.18 (12), p.3257-3266
Main Authors: Veeran, Midhu George, Jaya Sankar, Athira, Painuly, Diksha
Format: Article
Language:English
Subjects:
Analytical Chemistry
Biochemistry
Chemistry
Chemistry and Materials Science
Contraceptives
Correlation coefficients
Drug delivery systems
Drugs
Electrospinning
Entrapment
Formulations
High performance liquid chromatography
Inorganic Chemistry
Mathematical analysis
Nanofibers
Organic Chemistry
Original Paper
Parameters
Photodiodes
Physical Chemistry
Steroids
Sustained release
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Summary:Nanofibers-based drug delivery systems are promising alternative to formulate transdermal drug formulations, which are suitable for sustained release of dual drugs in combined drug systems. The parameters such as content of active ingredients and physical/chemical stability of a drug are essential to monitor in new drug delivery-based systems, especially with the low-dose formulations. It plays an important role in the development of new drug delivery systems under the category of nanomedicines. The objective of the study was to develop a simple, precise, authentic analytical method for the simultaneous estimation of both contraceptive steroids from coaxial electrospun fibers and to carry out the validation of the optimized method. The estimation of steroid drugs in pure form as well as from electrospun fiber sheet was performed by reverse-phase high-performance liquid chromatography (RP-HPLC). The method was optimized using different mobile phase combinations. The retention time for ethinylestradiol (EE) and levonorgestrel (LNG) was observed at 4.38 and 6.24 min, respectively, at a wavelength of 225 nm using photodiode array (PDA) detector. The method was found to be linear for both steroid drugs with correlation coefficients of 0.9996 and 0.9991 for LNG and EE, respectively. The method was specific for the simultaneous detection of both the drugs, with accuracy (95–100%) and precision (RSD 
ISSN:1735-207X
1735-2428
DOI:10.1007/s13738-021-02263-3