Conformational changes of high-molecular-weight DNA upon binding to noble metal nanoparticles in solution

Changes in the secondary and tertiary structure of DNA upon binding to silver, gold, and palladium nanoparticles (NPs) in solution were studied using UV spectroscopy, viscometry, dynamic light scattering, flow birefringence, and atomic force microscopy. A method for preparation of NP bioconjugates w...

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Bibliographic Details
Published in:Russian chemical bulletin 2021-09, Vol.70 (9), p.1718-1724
Main Authors: Kasyanenko, N. A., Baryshev, A. V., Bakulev, V. M.
Format: Article
Language:English
Subjects:
Anisotropy
Atomic force microscopy
Binding
Birefringence
Chemistry
Chemistry and Materials Science
Chemistry/Food Science
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Inorganic Chemistry
Nanoparticles
Noble metals
Organic Chemistry
Palladium
Photon correlation spectroscopy
Reduction (metal working)
Silver
Viscometry
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Summary:Changes in the secondary and tertiary structure of DNA upon binding to silver, gold, and palladium nanoparticles (NPs) in solution were studied using UV spectroscopy, viscometry, dynamic light scattering, flow birefringence, and atomic force microscopy. A method for preparation of NP bioconjugates with DNA was developed. The enhancement of optical anisotropy of the macromolecule upon its contacts with metal NPs was detected. The effect of the formed structures on the persistence length of DNA and on the volume effects in the system was analyzed. A mechanism of NP formation upon the reduction of DNA-bound metal ions was proposed.
ISSN:1066-5285
1573-9171
DOI:10.1007/s11172-021-3275-3