Molecular cloning and tissue expression analyses of a UF3GT gene from Capsicum annuum L

In the present study, a novel gene designated as UF3GT was isolated from Capsicum annuum L. Sequence and structural analysis determined that the UF3GT protein, which contained 1401 base pairs encodes 447 amino acids and belongs to the Glycosyltransferase-GTB-type superfamily. The isoelectric point a...

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Bibliographic Details
Published in:African journal of agricultural research 2013-07, Vol.8 (28), p.3695-3701
Main Authors: Hui, ZHOU, Ming hua, DENG, Jin fen, WEN, Jin long, HUO, Xue xiao, ZOU, Hai shan, ZHU
Format: Article
Language:English
Subjects:
Cloning
Genetics
Plant sciences
Science and Technology
Vegetables
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Summary:In the present study, a novel gene designated as UF3GT was isolated from Capsicum annuum L. Sequence and structural analysis determined that the UF3GT protein, which contained 1401 base pairs encodes 447 amino acids and belongs to the Glycosyltransferase-GTB-type superfamily. The isoelectric point and the molecular weight of this gene are 5.97 and 49589, respectively. The deduced amino acid sequence shows highly identity with known anthocyanin synthases in other species. The phylogenetic tree analysis revealed that the C. annuum L. UF3GT has a closer genetic relationship with the UF3GT of petunia (Petunia x hybrida). The RT-PCR gene expression analysis indicated that the UF3GT gene was mostly expressed in pericarp, moderately expressed in stem, leaf, flower, placenta and seed, whereas no expression signal was detected in root. The study established the primary foundation for further research on this C. annuum L. gene. [PUBLICATION ABSTRACT]
ISSN:1991-637X
1991-637X
DOI:10.5897/AJAR11.2056