Cytotoxicity assessment of Bio-C Repair Íon : A new calcium silicatebased cement

• Published in: Journal of dental research, dental clinics, dental prospects dental clinics, dental prospects, 2021-07, Vol.15 (3), p.152-156
• Main Authors: Klein-Junior, Celso Afonso, Zimmer, Roberto, Dobler, Tãnyre, Oliveira, Vanessa, Marinowic, Daniel Rodrigo, Özkömür, Ahmet, Reston, Eduardo Galia
• Format: Article
• Language: English
• Subjects: Antimicrobial activity; Biocompatibility; Biological activity; Calcium hydroxide; Cell culture; Cell signaling; Cell survival; Cement; Clinical trials; Cytotoxicity; Dental cement; Dental pulp; Dentistry; Fibroblasts; Inflammation; Mechanical properties; Mesenchyme; Stem cells; Variance analysis
• ISSN: 2008-210X; 2008-2118
• DOI: 10.34172/joddd.2021.026

According to ISO 10993-5: 2009 (E), Bio-C Repair lon+ exhibited mild and moderate cytotoxicity in the 24-hour and 7-day analyses, respectively. Keywords: Calcium silicate Cell survival Fibroblasts Mineral trioxide aggregate Introduction Advances in dentistry are associated with advances in dental materials and novel therapies that promote tissue regeneration without affecting the cell signaling processes mediated by undifferentiated mesenchymal cells.1 Direct pulp capping is a method designed to preserve the exposed dental pulp with a protective agent, inducing hard tissue repair.2 The protective materials used must have adequate biocompatibility and bioactivity to promote stem cell activity and pulp healing in permanent teeth.3 For many years, the material of choice for pulp exposure cases was calcium hydroxide until studies demonstrated better clinical performance of calcium silicate-based materials, such as mineral trioxide aggregate (MTA).4,5 Both materials have a similar mechanism of action; however, calcium silicate-based cements induce the formation of a more uniform and thick mineralized barrier, in addition to causing less inflammatory response and less pulp tissue necrosis.6 MTA cement has hydrophilic properties, good radiopacity, low solubility, high pH, antimicrobial activity, and the ability to expand after insertion in the place of interest.7-9 However, the material is difficult to handle for insertion and condensation in the cavity and has a long setting time.10,11 Due to good biological, physical, and mechanical properties, new versions of calcium silicate-based materials have been developed for use as capping materials. [...]this material has not been studied in vitro and in vivo, comparing its cytocompatibility with MTA Repair HP and Biodentine, which are materials widely used directly in pulp tissue. [...]the present study aimed to evaluate the toxic effects of four pulp capping calcium silicate-based cements on NIH 3T3 cells, of which the Bio-C Repair Íon+ is still in an experimental phase. According to ISO 10993-5: 2009 (E) (Table 3), in the 24-hour analysis, Bio-C Repair, Biodentine, and Bio-C Repair Ion+ demonstrated mild cytotoxicity, while MTA Repair HP exhibited moderate cytotoxicity.