Effect of Putrescine on Low-Temperature Acclimation in Chlamydomonas reinhardtii

• Published in: Phyton (Buenos Aires) 2022, Vol.91 (3), p.583-598
• Main Authors: S. Inal, Muslum, Unal, Dilek, Turkyilmaz Unal, Bengu, Ozturk, Munir
• Format: Article
• Language: English
• Subjects: Acclimation; Acclimatization; Algae; Antioxidants; Aquatic plants; Biosynthesis; Catalase; Chlamydomonas reinhardtii; Cold acclimation; Enzymatic activity; Gene expression; Heat shock proteins; Lipid peroxidation; Lipids; Low temperature; Metabolism; Peroxidation; Photosystem II; Physiological effects; Polyamines; Polymerase chain reaction; Proteins; Putrescine; Repair; Ribulose-bisphosphate carboxylase; Stress; Temperature
• ISSN: 1851-5657; 0031-9457; 1851-5657
• DOI: 10.32604/phyton.2022.018223

Putrescine is reported to be necessary for cold acclimation under low-temperature stress. In this study, the effect of low-temperature on some physiological and biochemical parameters has been investigated using the green algae Chlamydomonas reinhardtii. The lipid peroxidation rate, amount of Rubisco protein, activities of antioxidant enzymes and gene expression of polyamine biosynthesis (odc2, and spd1), heat shock proteins (hsp70c, hsp90a, and hsp90c), and PSII repair mechanisms (psba, rep27, and tba1) were determined to understand the low-temperature response. Exogenous putrescine application significantly increased Rubisco protein concentration and catalase enzyme activities under low-temperature stress. Moreover, real-time RT-PCR results and gene expression analysis showed that polyamine metabolism induced gene expression at low-temperatures in the first 24 h. In the same way, the gene expression of heat shock proteins (hsp70c, hsp90a, and hsp90c) decreased under low-temperature treatment for 72 h; however, application of putrescine enhanced the gene expression in the first 24 h. The results obtained indicated that molecular response in the first 24 h could be important for cold acclimation. The psba and tba1 expressions were reduced under low-temperatures depending on the exposure time. In contrast, the exogenous putrescine enhanced the expression level of the psba response to low-temperature at 24 and 72 h. The results obtained in this study indicate that putrescine could play a role in the PS II repair mechanisms under low-temperature stress.