Lutein exerts its cardioprotective effect against the experimental model of isoprenaline‐induced myocardial infarction via MIAT/miR‐200a/Nrf2/TXINP pathway

• Published in: Journal of biochemical and molecular toxicology 2021-11, Vol.35 (11), p.e22899-n/a
• Main Authors: Abdelmonem, Maha, Ibrahim, Sherehan M., Essam, Reham M., Amin, Hebat Allah A., Abd‐Elmawla, Mai A.
• Format: Article
• Language: English
• Subjects: Animals; Antioxidants; Calcium-binding protein; Cardiotonic Agents - pharmacology; Creatine; Desmin; Disease Models, Animal; Ejection fraction; Glutathione; Heart; Heart attacks; Inflammation; isoprenaline; Isoproterenol - toxicity; Lipid peroxidation; Lipids; Lutein; Lutein - pharmacology; MIAT; MicroRNAs - metabolism; miR‐200a; Molecular modelling; Morbidity; Myocardial infarction; Myocardial Infarction - chemically induced; Myocardial Infarction - metabolism; NF-E2-Related Factor 2 - metabolism; Nrf2; Peroxidation; Pretreatment; Rats; RNA, Long Noncoding - metabolism; Signal Transduction; Thioredoxin; Transcription; Troponin; Troponin T; TXNIP
• ISSN: 1095-6670; 1099-0461
• DOI: 10.1002/jbt.22899

Acute myocardial infarction (AMI) remains a leading cause of morbidity and mortality worldwide. Lutein (LU) possesses numerous pharmacological activities, including anti‐inflammatory, antioxidant, and antiapoptotic effects. This study aimed to investigate the cardioprotective potential of LU in isoprenaline (ISO)‐induced MI and to explore its molecular mechanisms of action. AMI was induced by two consecutive subcutaneous doses of ISO (65 mg/kg; s.c.). The LU group was pretreated with LU (20 mg/kg; p.o.) for 30 days followed by ISO injections on Days 29 and 30. ISO group showed elevated serum creatine kinas‐MB (CK‐MB) and considerable electrocardiographic changes along with reduced ejection fraction compared to the normal group. LU pretreatment could decrease serum CK‐MB activity, normalize QRS and QTc intervals and restore ejection fraction compared to the untreated group. The ISO group demonstrated infarcted‐like lesions, which were ameliorated in the LU‐pretreated group. Immunohistochemical investigation revealed upregulated cardiac troponin T (cTn T) and desmin expressions in the LU‐pretreated group. LU pretreatment also enhanced cardiac thioredoxin (Trx) and glutathione (GSH) contents as well as reduced lipid peroxidation, compared to the untreated group. Importantly, LU pretreatment could downregulate long noncoding MI associated transcript (lncRNA MIAT) and thioredoxin‐interacting protein (TXNIP) and augment micro RNA (miR)‐200a and nuclear factor erythroid 2‐related factor 2 (Nrf2) expressions compared to the ISO group. Moreover, a significant inverse correlation between MIAT and miR‐200a was observed. In conclusion, this study revealed that LU could ameliorate ISO‐induced MI in rats by modulating MIAT/miR‐200a/Nrf2 pathway.