Coupling smartphone and CRISPR–Cas12a for digital and multiplexed nucleic acid detection

Accurate, rapid, and multiplexed nucleic acid detection is of great value for applications in biomedicine and agriculture. Here, we demonstrated a one‐step Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) diagnostic method for the digital and multiplexed detection of both single‐st...

Full description

Saved in:
Bibliographic Details
Published in:AIChE journal 2021-12, Vol.67 (12), p.n/a
Main Authors: Yu, Tao, Zhang, Shengwei, Matei, Razvan, Marx, William, Beisel, Chase L., Wei, Qingshan
Format: Article
Language:English
Subjects:
Assaying
Cas12a
CRISPR
CRISPR diagnostics
Deoxyribonucleic acid
Diagnostic software
digital assay
DNA
DNA viruses
Hepatitis B
Human papillomavirus
Human performance
Multiplexing
Nucleic acids
point‐of‐care diagnostics
Reaction kinetics
smartphone device
Smartphones
Target detection
Viruses
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c2975-ecb9d273ce11dd95fd946fcaa6f6c4f64555a3ed83297eda87b9d04e8cc5b1de3
cites cdi_FETCH-LOGICAL-c2975-ecb9d273ce11dd95fd946fcaa6f6c4f64555a3ed83297eda87b9d04e8cc5b1de3
container_end_page n/a
container_issue 12
container_start_page
container_title AIChE journal
container_volume 67
creator Yu, Tao
Zhang, Shengwei
Matei, Razvan
Marx, William
Beisel, Chase L.
Wei, Qingshan
description Accurate, rapid, and multiplexed nucleic acid detection is of great value for applications in biomedicine and agriculture. Here, we demonstrated a one‐step Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) diagnostic method for the digital and multiplexed detection of both single‐stranded (ss) and double‐stranded (ds) DNA on a handheld smartphone device. ssDNA targets exhibited faster reaction kinetics of this single‐step CRISPR–Cas12a assay than dsDNA counterparts. Under optimized conditions, picomolar levels of ssDNA targets can be detected in 96‐well plates by using a benchtop plate reader. The detection sensitivity can be further improved to 5 fM when running the reaction in a microwell‐based digital assay chip. Multiplexed detection of hepatitis B virus and human papillomavirus DNA markers was demonstrated on the smartphone‐based platform. Finally, the one‐step CRISPR–Cas12a assay performed robustly in human serum samples with a recovery rate of ssDNA detection between 96% and 105.6%, suggesting a high potential for clinical diagnostic applications in point‐of‐care settings.
doi_str_mv 10.1002/aic.17365
format article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_2597408849</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2597408849</sourcerecordid><originalsourceid>FETCH-LOGICAL-c2975-ecb9d273ce11dd95fd946fcaa6f6c4f64555a3ed83297eda87b9d04e8cc5b1de3</originalsourceid><addsrcrecordid>eNp1kL1OwzAQgC0EEqUw8AaWmBjS2o6dn7GKoFSqBCqwsFiufS6u0iTEiaAb78Ab8iSYhpXpdHff3ek-hC4pmVBC2FQ5PaFpnIgjNKKCp5HIiThGI0IIjUKBnqIz77chY2nGRuilqPumdNUG-51qu-a1rgCryuBitXh8WH1_fhXKU6awrVts3MZ1qjz0d33ZuaaEDzC46nUJTmOlncEGOtCdq6tzdGJV6eHiL47R8-3NU3EXLe_ni2K2jDTLUxGBXueGpbEGSo3JhTU5T6xWKrGJ5jbhQggVg8nigINRWRp4wiHTWqypgXiMroa9TVu_9eA7ua37tgonJRN5ykmW8TxQ1wOl29r7FqxsWhde3ktK5K86GdTJg7rATgf23ZWw_x-Us0UxTPwATGRxXg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2597408849</pqid></control><display><type>article</type><title>Coupling smartphone and CRISPR–Cas12a for digital and multiplexed nucleic acid detection</title><source>Wiley-Blackwell Read &amp; Publish Collection</source><creator>Yu, Tao ; Zhang, Shengwei ; Matei, Razvan ; Marx, William ; Beisel, Chase L. ; Wei, Qingshan</creator><creatorcontrib>Yu, Tao ; Zhang, Shengwei ; Matei, Razvan ; Marx, William ; Beisel, Chase L. ; Wei, Qingshan</creatorcontrib><description>Accurate, rapid, and multiplexed nucleic acid detection is of great value for applications in biomedicine and agriculture. Here, we demonstrated a one‐step Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) diagnostic method for the digital and multiplexed detection of both single‐stranded (ss) and double‐stranded (ds) DNA on a handheld smartphone device. ssDNA targets exhibited faster reaction kinetics of this single‐step CRISPR–Cas12a assay than dsDNA counterparts. Under optimized conditions, picomolar levels of ssDNA targets can be detected in 96‐well plates by using a benchtop plate reader. The detection sensitivity can be further improved to 5 fM when running the reaction in a microwell‐based digital assay chip. Multiplexed detection of hepatitis B virus and human papillomavirus DNA markers was demonstrated on the smartphone‐based platform. Finally, the one‐step CRISPR–Cas12a assay performed robustly in human serum samples with a recovery rate of ssDNA detection between 96% and 105.6%, suggesting a high potential for clinical diagnostic applications in point‐of‐care settings.</description><identifier>ISSN: 0001-1541</identifier><identifier>EISSN: 1547-5905</identifier><identifier>DOI: 10.1002/aic.17365</identifier><language>eng</language><publisher>Hoboken, USA: John Wiley &amp; Sons, Inc</publisher><subject>Assaying ; Cas12a ; CRISPR ; CRISPR diagnostics ; Deoxyribonucleic acid ; Diagnostic software ; digital assay ; DNA ; DNA viruses ; Hepatitis B ; Human papillomavirus ; Human performance ; Multiplexing ; Nucleic acids ; point‐of‐care diagnostics ; Reaction kinetics ; smartphone device ; Smartphones ; Target detection ; Viruses</subject><ispartof>AIChE journal, 2021-12, Vol.67 (12), p.n/a</ispartof><rights>2021 American Institute of Chemical Engineers.</rights><rights>2021 American Institute of Chemical Engineers</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c2975-ecb9d273ce11dd95fd946fcaa6f6c4f64555a3ed83297eda87b9d04e8cc5b1de3</citedby><cites>FETCH-LOGICAL-c2975-ecb9d273ce11dd95fd946fcaa6f6c4f64555a3ed83297eda87b9d04e8cc5b1de3</cites><orcidid>0000-0003-1214-010X ; 0000-0003-0650-9943</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids></links><search><creatorcontrib>Yu, Tao</creatorcontrib><creatorcontrib>Zhang, Shengwei</creatorcontrib><creatorcontrib>Matei, Razvan</creatorcontrib><creatorcontrib>Marx, William</creatorcontrib><creatorcontrib>Beisel, Chase L.</creatorcontrib><creatorcontrib>Wei, Qingshan</creatorcontrib><title>Coupling smartphone and CRISPR–Cas12a for digital and multiplexed nucleic acid detection</title><title>AIChE journal</title><description>Accurate, rapid, and multiplexed nucleic acid detection is of great value for applications in biomedicine and agriculture. Here, we demonstrated a one‐step Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) diagnostic method for the digital and multiplexed detection of both single‐stranded (ss) and double‐stranded (ds) DNA on a handheld smartphone device. ssDNA targets exhibited faster reaction kinetics of this single‐step CRISPR–Cas12a assay than dsDNA counterparts. Under optimized conditions, picomolar levels of ssDNA targets can be detected in 96‐well plates by using a benchtop plate reader. The detection sensitivity can be further improved to 5 fM when running the reaction in a microwell‐based digital assay chip. Multiplexed detection of hepatitis B virus and human papillomavirus DNA markers was demonstrated on the smartphone‐based platform. Finally, the one‐step CRISPR–Cas12a assay performed robustly in human serum samples with a recovery rate of ssDNA detection between 96% and 105.6%, suggesting a high potential for clinical diagnostic applications in point‐of‐care settings.</description><subject>Assaying</subject><subject>Cas12a</subject><subject>CRISPR</subject><subject>CRISPR diagnostics</subject><subject>Deoxyribonucleic acid</subject><subject>Diagnostic software</subject><subject>digital assay</subject><subject>DNA</subject><subject>DNA viruses</subject><subject>Hepatitis B</subject><subject>Human papillomavirus</subject><subject>Human performance</subject><subject>Multiplexing</subject><subject>Nucleic acids</subject><subject>point‐of‐care diagnostics</subject><subject>Reaction kinetics</subject><subject>smartphone device</subject><subject>Smartphones</subject><subject>Target detection</subject><subject>Viruses</subject><issn>0001-1541</issn><issn>1547-5905</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><recordid>eNp1kL1OwzAQgC0EEqUw8AaWmBjS2o6dn7GKoFSqBCqwsFiufS6u0iTEiaAb78Ab8iSYhpXpdHff3ek-hC4pmVBC2FQ5PaFpnIgjNKKCp5HIiThGI0IIjUKBnqIz77chY2nGRuilqPumdNUG-51qu-a1rgCryuBitXh8WH1_fhXKU6awrVts3MZ1qjz0d33ZuaaEDzC46nUJTmOlncEGOtCdq6tzdGJV6eHiL47R8-3NU3EXLe_ni2K2jDTLUxGBXueGpbEGSo3JhTU5T6xWKrGJ5jbhQggVg8nigINRWRp4wiHTWqypgXiMroa9TVu_9eA7ua37tgonJRN5ykmW8TxQ1wOl29r7FqxsWhde3ktK5K86GdTJg7rATgf23ZWw_x-Us0UxTPwATGRxXg</recordid><startdate>202112</startdate><enddate>202112</enddate><creator>Yu, Tao</creator><creator>Zhang, Shengwei</creator><creator>Matei, Razvan</creator><creator>Marx, William</creator><creator>Beisel, Chase L.</creator><creator>Wei, Qingshan</creator><general>John Wiley &amp; Sons, Inc</general><general>American Institute of Chemical Engineers</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7ST</scope><scope>7U5</scope><scope>8FD</scope><scope>C1K</scope><scope>L7M</scope><scope>SOI</scope><orcidid>https://orcid.org/0000-0003-1214-010X</orcidid><orcidid>https://orcid.org/0000-0003-0650-9943</orcidid></search><sort><creationdate>202112</creationdate><title>Coupling smartphone and CRISPR–Cas12a for digital and multiplexed nucleic acid detection</title><author>Yu, Tao ; Zhang, Shengwei ; Matei, Razvan ; Marx, William ; Beisel, Chase L. ; Wei, Qingshan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2975-ecb9d273ce11dd95fd946fcaa6f6c4f64555a3ed83297eda87b9d04e8cc5b1de3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Assaying</topic><topic>Cas12a</topic><topic>CRISPR</topic><topic>CRISPR diagnostics</topic><topic>Deoxyribonucleic acid</topic><topic>Diagnostic software</topic><topic>digital assay</topic><topic>DNA</topic><topic>DNA viruses</topic><topic>Hepatitis B</topic><topic>Human papillomavirus</topic><topic>Human performance</topic><topic>Multiplexing</topic><topic>Nucleic acids</topic><topic>point‐of‐care diagnostics</topic><topic>Reaction kinetics</topic><topic>smartphone device</topic><topic>Smartphones</topic><topic>Target detection</topic><topic>Viruses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yu, Tao</creatorcontrib><creatorcontrib>Zhang, Shengwei</creatorcontrib><creatorcontrib>Matei, Razvan</creatorcontrib><creatorcontrib>Marx, William</creatorcontrib><creatorcontrib>Beisel, Chase L.</creatorcontrib><creatorcontrib>Wei, Qingshan</creatorcontrib><collection>CrossRef</collection><collection>Environment Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Environment Abstracts</collection><jtitle>AIChE journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yu, Tao</au><au>Zhang, Shengwei</au><au>Matei, Razvan</au><au>Marx, William</au><au>Beisel, Chase L.</au><au>Wei, Qingshan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Coupling smartphone and CRISPR–Cas12a for digital and multiplexed nucleic acid detection</atitle><jtitle>AIChE journal</jtitle><date>2021-12</date><risdate>2021</risdate><volume>67</volume><issue>12</issue><epage>n/a</epage><issn>0001-1541</issn><eissn>1547-5905</eissn><abstract>Accurate, rapid, and multiplexed nucleic acid detection is of great value for applications in biomedicine and agriculture. Here, we demonstrated a one‐step Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) diagnostic method for the digital and multiplexed detection of both single‐stranded (ss) and double‐stranded (ds) DNA on a handheld smartphone device. ssDNA targets exhibited faster reaction kinetics of this single‐step CRISPR–Cas12a assay than dsDNA counterparts. Under optimized conditions, picomolar levels of ssDNA targets can be detected in 96‐well plates by using a benchtop plate reader. The detection sensitivity can be further improved to 5 fM when running the reaction in a microwell‐based digital assay chip. Multiplexed detection of hepatitis B virus and human papillomavirus DNA markers was demonstrated on the smartphone‐based platform. Finally, the one‐step CRISPR–Cas12a assay performed robustly in human serum samples with a recovery rate of ssDNA detection between 96% and 105.6%, suggesting a high potential for clinical diagnostic applications in point‐of‐care settings.</abstract><cop>Hoboken, USA</cop><pub>John Wiley &amp; Sons, Inc</pub><doi>10.1002/aic.17365</doi><tpages>13</tpages><orcidid>https://orcid.org/0000-0003-1214-010X</orcidid><orcidid>https://orcid.org/0000-0003-0650-9943</orcidid></addata></record>
fulltext fulltext
identifier ISSN: 0001-1541
ispartof AIChE journal, 2021-12, Vol.67 (12), p.n/a
issn 0001-1541
1547-5905
language eng
recordid cdi_proquest_journals_2597408849
source Wiley-Blackwell Read & Publish Collection
subjects Assaying
Cas12a
CRISPR
CRISPR diagnostics
Deoxyribonucleic acid
Diagnostic software
digital assay
DNA
DNA viruses
Hepatitis B
Human papillomavirus
Human performance
Multiplexing
Nucleic acids
point‐of‐care diagnostics
Reaction kinetics
smartphone device
Smartphones
Target detection
Viruses
title Coupling smartphone and CRISPR–Cas12a for digital and multiplexed nucleic acid detection
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-30T22%3A29%3A27IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Coupling%20smartphone%20and%20CRISPR%E2%80%93Cas12a%20for%20digital%20and%20multiplexed%20nucleic%20acid%20detection&rft.jtitle=AIChE%20journal&rft.au=Yu,%20Tao&rft.date=2021-12&rft.volume=67&rft.issue=12&rft.epage=n/a&rft.issn=0001-1541&rft.eissn=1547-5905&rft_id=info:doi/10.1002/aic.17365&rft_dat=%3Cproquest_cross%3E2597408849%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c2975-ecb9d273ce11dd95fd946fcaa6f6c4f64555a3ed83297eda87b9d04e8cc5b1de3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=2597408849&rft_id=info:pmid/&rfr_iscdi=true