Heparin Gold Nanoparticles as a Colorimetric Probe for Cardiac Troponin I Detection

We report a simple, visual detection strategy for cardiac biomarker, troponin I ( cTnI ), a promising indicator of cardiac disease based on heparin gold nanoparticles ( HAuNPs ). The red shift in the plasmon absorption band followed by color change of the solution is the basic principle of the assay...

Full description

Saved in:
Bibliographic Details
Published in:Journal of analytical chemistry (New York, N.Y.) N.Y.), 2021-11, Vol.76 (11), p.1245-1251
Main Authors: Raj, Vidya, Alex, Saji
Format: Article
Language:English
Subjects:
Absorption spectra
Analytical Chemistry
Antibodies
Binding
Biomarkers
Blood proteins
Cardiovascular diseases
Chemistry
Chemistry and Materials Science
Color
Colorimetry
Doppler effect
Gold
Heparin
Investigations
Nanoparticles
Red shift
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We report a simple, visual detection strategy for cardiac biomarker, troponin I ( cTnI ), a promising indicator of cardiac disease based on heparin gold nanoparticles ( HAuNPs ). The red shift in the plasmon absorption band followed by color change of the solution is the basic principle of the assay. The observed red shift and color change were attributed to the strong electrostatic attraction between the glycoaminoglycans and heparin binding groups present in cTnI. The method exhibits linearity between cTnI concentration and corresponding absorbance in the range of 0.5–20 ng/mL with a detection limit of 0.3 ng/mL. Other major blood proteins did not show any interference, and the binding was found to be pH dependent. The antibody free method was successfully applied to the quantification of cTnI in human serum and showed good correlation with the hospital measurements. Thus, HAuNPs-based assay is a quick, label-free platform for cTnI detection.
ISSN:1061-9348
1608-3199
DOI:10.1134/S1061934821110113