Optimization of Microfluidics for Genetic Analysis

Novel microfluidic designs are needed for fluid delivery and transport in an integrated micro-genetic analysis system and these designs often lead to complex relationships between the shape and size of the microchannels and the behavior of multi-component fluids. We are studying both pressure-driven...

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Bibliographic Details
Published in:Microscopy and microanalysis 1998-07, Vol.4 (S2), p.892-893
Main Authors: St. John, Pamela M., Connell, Kip, Woudenberg, T. M., Deshpande, M., Gilbert, J. R.
Format: Article
Language:English
Subjects:
Design
Dyes
Electrokinetics
Flow profiles
Fluorescence
Genetic analysis
Glass substrates
Irradiation
Microchannels
Microfluidics
Miniaturized Artificial Machines in Biology
Neodymium lasers
Optimization
Semiconductor lasers
Silicones
YAG lasers
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Summary:Novel microfluidic designs are needed for fluid delivery and transport in an integrated micro-genetic analysis system and these designs often lead to complex relationships between the shape and size of the microchannels and the behavior of multi-component fluids. We are studying both pressure-driven and electrokinetic flow in various microfluidic channel designs fabricated from both glass and silicone substrates to understand chemical containment and transport. In conjunction with the experiments, simulations are being performed to predict behavior in microdevices and models are being used to develop a CAD tool for designers. Fluorescent flow profiles are generated using a charged caged dye which fluoresces upon irradiation with 355 nm light from a Nd:YAG laser. The starting position of the dye is determined by a tightly focused laser spot
ISSN:1431-9276
1435-8115
DOI:10.1017/S1431927600024582