Molecular characterization and antibacterial activity of oral antibiotics and copper nanoparticles against endodontic pathogens commonly related to health care-associated infections

Objective To carry out molecular characterization and determine the antibacterial activity of oral antibiotics and copper nanoparticles (Cu-NPs) against endodontic strains isolated from persistent infections. Materials and methods Root canal samples from 24 teeth in different patients with persisten...

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Bibliographic Details
Published in:Clinical oral investigations 2021-12, Vol.25 (12), p.6729-6741
Main Authors: Sacoto-Figueroa, Fernanda Katherine, Bello-Toledo, Helia Magali, González-Rocha, Gerardo Enrique, Luengo Machuca, Luis, Lima, Celia A., Meléndrez-Castro, Manuel, Sánchez-Sanhueza, Gabriela Alejandra
Format: Article
Language:English
Subjects:
Antibacterial activity
Antibiotics
Clarithromycin
Copper
Dentistry
Erythromycin
Genotyping
Health care
Infections
Kruskal-Wallis test
Medicine
Metronidazole
Minimum inhibitory concentration
Nanoparticles
Nosocomial infections
Original Article
Patients
Persistent infection
Pseudomonas
Root canals
rRNA 16S
Strains (organisms)
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Summary:Objective To carry out molecular characterization and determine the antibacterial activity of oral antibiotics and copper nanoparticles (Cu-NPs) against endodontic strains isolated from persistent infections. Materials and methods Root canal samples from 24 teeth in different patients with persistent endodontic infections were obtained. The isolated strains were identified by biochemical tests and 16S rDNA sequencing. Genotyping was achieved by molecular methods. The antibacterial activity of antibiotics and copper nanostructures was determined by using minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values. Furthermore, a time-kill kinetics assay was evaluated. Nonparametric tests (Kruskal-Wallis ANOVA) were performed ( p value
ISSN:1432-6981
1436-3771
DOI:10.1007/s00784-021-03959-9