Content of apoptosis factors and self-organization processes in the mitochondria of heart cells in female mice C57BL/6 under growth of melanoma B16 / F10 linked with comorbid pathology

The aim is to study some mechanisms of regulation of apoptosis and self-organization in the mitochondria in the heart cells in female mice during the growth of experimental melanoma B16/ F10 linked with chronic neurogenic pain as comorbid pathology. Materials and methods The work has been performed...

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Bibliographic Details
Published in:Cardiometry 2021-05 (18), p.121-130
Main Authors: Frantsiyants, Elena M, Neskubina, Irina V, Shikhlyarova, Alla I, Yengibaryan, Marina A, Vashchenko, Larisa N, Surikova, Ekaterina I, Nemashkalova, Lyudmila A, Kaplieva, Irina V, Trepitaki, Lidia K, Bandovkina, Valeriya A, Pogorelova, Yulia A
Format: Article
Language:English
Subjects:
Apoptosis
Comorbidity
Cytochrome
Heart
Melanoma
Mitochondria
Pathology
Proteins
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Summary:The aim is to study some mechanisms of regulation of apoptosis and self-organization in the mitochondria in the heart cells in female mice during the growth of experimental melanoma B16/ F10 linked with chronic neurogenic pain as comorbid pathology. Materials and methods The work has been performed in female mice of the C57BL/6 strain (n=168).The following experimental groups have been presented: the group of intact mice (n=21), the control group (n=21) to reproduce chronic neurogenic pain (CNP), the comparison group (n=63) with a standard growth of melanoma B16/F10 and the main group (n=63) with CNP + melanoma B16 / F10. In mitochondria the following concentrations have been determined by the ELISA method: cytochrome C (ng/mg protein), caspase-9 (ng/mg protein), Bcl-2 (ng/mg protein), AIF (ng/mg protein) and calcium (mmol/g protein). The Statistica 10.0 software was used for statistical analysis. Results As against the intact values, the standard growth of melanoma reduced in the mitochondria of the heart cells the level of calcium in week 1-2 by 2.9 and 6.7 times, respectively, the AIF content in week 2-3 by 1.4 times (p < 0.05) and 2 times, respectively; Bcl-2 after 1-2 weeks by 1.7 and 2.9 times, while cytochrome C and caspase 9 remained stable throughout the entire period of the melanoma growth. CNP induced a decrease in the level of Ca2+ by 3.2 times, that in Bcl-2 by 1.3 times (p
ISSN:2304-7232
DOI:10.18137/cardiometry.2021.18.121130