Development of Immunochromatographic Test System for Detection of Antibiotic Clinafloxacin and Its Application for Honey Control

To ensure express control of food quality and safety, we developed an immunochromatographic test system for detection of the antibiotic clinafloxacin. The test system implements an indirect competitive immunoassay format based on the interaction of unmodified specific polyclonal antibodies with the...

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Bibliographic Details
Published in:Applied biochemistry and microbiology 2021-11, Vol.57 (6), p.778-785
Main Authors: Zvereva, E. A., Sotnikov, D. V., Belichenko, K. A., Hendrickson, O. D., Shanin, I. A., Zherdev, A. V., Dzantiev, B. B.
Format: Article
Language:English
Subjects:
Antibiotics
Antibodies
Antigen-antibody complexes
Biochemistry
Biomedical and Life Sciences
Clinafloxacin
Conjugates
Detection limits
Fluoroquinolones
Food contamination
Food quality
Food safety
Honey
Immunoassay
Life Sciences
Medical Microbiology
Membranes
Microbiology
Nanoparticles
Pollution control
Polyclonal antibodies
Sample preparation
Selectivity
Test systems
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Summary:To ensure express control of food quality and safety, we developed an immunochromatographic test system for detection of the antibiotic clinafloxacin. The test system implements an indirect competitive immunoassay format based on the interaction of unmodified specific polyclonal antibodies with the antibiotic potentially present in the sample and the antibiotic–protein conjugate immobilized on the working membrane of the test strip. The detection of immune complexes formed on the membrane is carried out using a conjugate of anti-species antibodies and gold nanoparticles. We determined immunochromatography conditions that ensure the achievement of the minimum detection limit. At the assay duration of 20 min, the instrumental and visual detection limits of clinafloxacin comprise 0.3 and 10 ng/mL, respectively. The selectivity of the test system in relation to antibiotics from the class of fluoroquinolones was studied. To ensure the control of the contamination of honey with clinafloxacin and structurally related fluoroquinolones, we identified an optimal sample preparation regimen and confirmed the efficacy of the developed test system.
ISSN:0003-6838
1608-3024
DOI:10.1134/S0003683821060144