One-pot biocatalytic synthesis of nylon monomers from cyclohexanol using Escherichia coli -based concurrent cascade consortia

We developed a multienzyme biocatalytic cascade with high atom efficiency and a self-sufficient redox network for the synthesis of nylon monomers without adding auxiliary enzymes to recycle cofactors. Herein, a cell-based modularization strategy was applied to produce 6-aminohexanoic acid and ε-capr...

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Bibliographic Details
Published in:Green chemistry : an international journal and green chemistry resource : GC 2021-11, Vol.23 (23), p.9447-9453
Main Authors: Sarak, Sharad, Khobragade, Taresh P., Jeon, Hyunwoo, Pagar, Amol D., Giri, Pritam, Lee, Somin, Yun, Hyungdon
Format: Article
Language:English
Subjects:
Atom economy
Caprolactam
Cofactors
Consortia
Cyclohexanol
E coli
Green chemistry
Monomers
Nylon
Substrates
Synthesis
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Summary:We developed a multienzyme biocatalytic cascade with high atom efficiency and a self-sufficient redox network for the synthesis of nylon monomers without adding auxiliary enzymes to recycle cofactors. Herein, a cell-based modularization strategy was applied to produce 6-aminohexanoic acid and ε-caprolactam from cyclohexanol. The designed consortia of E. coli containing the respective modules was found to produce >97% of 6-aminohexanoic acid (from 100 mM cyclohexanol) and 28% of caprolactam (from 10 mM cyclohexanol) with 2-fold excess of propylamine in a one-pot reaction. The use of cheaper substrates and co-substrates makes it an economically beneficial approach for the production of nylon precursors.
ISSN:1463-9262
1463-9270
DOI:10.1039/D1GC03056F