An ultrasensitive electrochemiluminescence assay for nucleic acid detection based on carboxyl functionalized polymer dots

•This paper proposed an ECL biosensor based on polymer dots for ultrasensitive detection of miRNA-122.•A novel carboxyl functionalized Pdot was prepared by nanoprecipitation without exogenous functionalized reagents.•The biosensor employed two-step signal amplification strategy including enzyme assi...

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Bibliographic Details
Published in:Journal of electroanalytical chemistry (Lausanne, Switzerland) Switzerland), 2021-11, Vol.900, p.115743, Article 115743
Main Authors: Zhang, Nan, Zhao, Zi-Yuan, Gao, Hang, Yu, Yue, Pan, Jian-Bin, Chen, Hong-Yuan, Xu, Jing-Juan
Format: Article
Language:English
Subjects:
Amplification
Biosensors
Carboxyl group
Electrochemiluminescence
Electrochemiluminescence bioassay
Emitters
MicroRNA-122
Nuclease
Nucleic acids
Polymer dots
Polymers
Ultrasensitive detection
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Summary:•This paper proposed an ECL biosensor based on polymer dots for ultrasensitive detection of miRNA-122.•A novel carboxyl functionalized Pdot was prepared by nanoprecipitation without exogenous functionalized reagents.•The biosensor employed two-step signal amplification strategy including enzyme assisted recycle and rolling circle amplification.•This work will further expand the applications of Pdots nanomaterials in the ECL bioanalysis. An electrochemiluminescence (ECL) sensing platform for ultrasensitive detection of miRNA-122 was developed by integrating novel emitters of polymer dots (Pdots) and signal amplification strategies. Firstly, we synthesized a novel polymer with carboxyl group which consisted of fluorene derivate and benzothiadiazole, corresponding Pdots materials were also prepared by nanoprecipitation method. Subsequently, an ECL biosensor based on the Pdots for miRNA-122 detection was constructed, which combines the amplification of duplex-specific nuclease (DSN)-assisted target recycling and rolling circle amplification (RCA) in-situ on the electrode. After the two-step amplification, amounts of ferrocene modified DNA (Fc-DNA) were captured on the electrode, which could quench the ECL signal of the Pdots. Ultimately, the proposed sensor presented a wide linear range of 0.1 fM to 100 pM and a low detection limit of 36 aM to miRNA-122. This work not only propose a universal paradigm for nucleic acid detection with good specificity and high sensitivity, but also provide an approach for designing novel Pdots to construct ECL sensor conveniently.
ISSN:1572-6657
1873-2569
DOI:10.1016/j.jelechem.2021.115743