The composites of asymmetric Co phthalocyanines-graphitic carbon nitride quantum dots-aptamer as specific electrochemical sensors for the detection of prostate specific antigen: Effects of ring substituents

•.Aptamer - phthalocyanine - quantum dots are used as sensors for prostate antigen.•Electrochemical impedance spectroscopy is employed to determine detection limits.•Low detection limit of 0.27 ng/mL in real sample. Asymmetrical phthalocaynine molecules have been employed in combination with graphit...

Full description

Saved in:
Bibliographic Details
Published in:Journal of electroanalytical chemistry (Lausanne, Switzerland) Switzerland), 2021-11, Vol.900, p.115730, Article 115730
Main Authors: Robin Nxele, Siphesihle, Nkhahle, Reitumetse, Nyokong, Tebello
Format: Article
Language:English
Subjects:
Antigens
Aptamer
Aptasensor
Asymmetrical phthalocyanines
Asymmetry
Biomarkers
Buffer solutions
Carbon nitride
Chemical sensors
Electrochemical analysis
Electrons
Graphitic carbon nitride quantum dots
Prostate cancer
Prostate specific antigen
Quantum dots
Reproducibility
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:•.Aptamer - phthalocyanine - quantum dots are used as sensors for prostate antigen.•Electrochemical impedance spectroscopy is employed to determine detection limits.•Low detection limit of 0.27 ng/mL in real sample. Asymmetrical phthalocaynine molecules have been employed in combination with graphitic carbon nitride quantum dots covalently linked to an amine-functionalized aptamer for the electrochemical detection of prostate cancer biomarker. The combination of the phthalocyanine complexes with the quantum dots-aptamer conjugate resulted in an overall improved electrocatalytic ability as well as enhanced signal response. Moreover, results proved one of the complexes being superior over the other functionality wise, indicating the significance of substituent type of the phthalocyanines. The best performing aptasensor showed detection limits as low as 0.23 ng/mL in buffer solutions as well as 0.27 ng/mL in serum samples. Moreover, the sensor showed good stability, reproducibility and repeatability.
ISSN:1572-6657
1873-2569
DOI:10.1016/j.jelechem.2021.115730