Optimization of a decellularization protocol of porcine tracheas. Long-term effects of cryopreservation. A histological study

Objective: The aim of this study was to optimize a decellularization protocol in the trachea of Sus scrofa domestica (pig) as well as to study the effects of long-term cryopreservation on the extracellular matrix of decellularized tracheas. Methods: Porcine tracheas were decellularized using Triton...

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Bibliographic Details
Published in:International journal of artificial organs 2021-12, Vol.44 (12), p.998-1012
Main Authors: Milian, Lara, Sancho-Tello, María, Roig-Soriano, Joan, Foschini, Giovanna, Martínez-Hernández, Néstor J, Más-Estellés, Jorge, Ruiz-Sauri, Amparo, Zurriaga, Javier, Carda, Carmen, Mata, Manuel
Format: Article
Language:English
Subjects:
Animals
Biocompatibility
Biomechanics
Chondrocytes
Collagen
Cryopreservation
Detergents
Extracellular Matrix
Fibers
Long-term effects
Mechanical properties
Octoxynol
Optimization
Organs
Swine
Tissue Engineering
Tissue Scaffolds
Trachea
Ultrastructure
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Summary:Objective: The aim of this study was to optimize a decellularization protocol in the trachea of Sus scrofa domestica (pig) as well as to study the effects of long-term cryopreservation on the extracellular matrix of decellularized tracheas. Methods: Porcine tracheas were decellularized using Triton X-100, SDC, and SDS alone or in combination. The effect of these detergents on the extracellular matrix characteristics of decellularized porcine tracheas was evaluated at the histological, biomechanical, and biocompatibility level. Morphometric approaches were used to estimate the effect of detergents on the collagen and elastic fibers content as well as on the removal of chondrocytes from decellularized organs. Moreover, the long-term structural, ultrastructural, and biomechanical effect of cryopreservation of decellularized tracheas were also estimated. Results: Two percent SDS was the most effective detergent tested concerning cell removal and preservation of the histological and biomechanical properties of the tracheal wall. However, long-term cryopreservation had no an appreciable effect on the structure, ultrastructure, and biomechanics of decellularized tracheal rings. Conclusion: The results presented here reinforce the use of SDS as a valuable decellularizing agent for porcine tracheas. Furthermore, a cryogenic preservation protocol is described, which has minimal impact on the histological and biomechanical properties of decellularized porcine tracheas.
ISSN:0391-3988
1724-6040
DOI:10.1177/03913988211008912