Advanced strategies and tools to facilitate and streamline microbial adaptive laboratory evolution

Adaptive laboratory evolution (ALE) has served as a historic microbial engineering method that mimics natural selection to obtain desired microbes. The past decade has witnessed improvements in all aspects of ALE workflow, in terms of growth coupling, genotypic diversification, phenotypic selection,...

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Bibliographic Details
Published in:Trends in biotechnology (Regular ed.) 2022-01, Vol.40 (1), p.38-59
Main Authors: Wu, Yinan, Jameel, Aysha, Xing, Xin-Hui, Zhang, Chong
Format: Article
Language:English
Subjects:
adaptive laboratory evolution
Assimilation
Automation
Biosensors
Biosynthesis
Carbon
Coupling
E coli
Enzymes
Evolution
Gene mapping
Genes
Genetic analysis
Genetic Engineering
Genomes
Genotype
Genotype & phenotype
Genotypes
growth-coupling strategy
Homocysteine
In vivo methods and tests
in vivo mutagenesis method
Metabolism
Metabolites
microbial engineering
Microorganisms
multi-omics analysis
multiplexed automated culture platform
Multiplexing
Mutagenesis
Mutation
Natural selection
Nutrition
Phenotype
Phenotypes
Workflow
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Summary:Adaptive laboratory evolution (ALE) has served as a historic microbial engineering method that mimics natural selection to obtain desired microbes. The past decade has witnessed improvements in all aspects of ALE workflow, in terms of growth coupling, genotypic diversification, phenotypic selection, and genotype–phenotype mapping. The developing growth-coupling strategies facilitate ALE to a wider range of appealing traits. In vivo mutagenesis methods and multiplexed automated culture platforms open new gates to streamline its execution. Meanwhile, the application of multi-omics analyses and multiplexed genetic engineering promote efficient knowledge mining. This article provides a comprehensive and updated review of these advances, highlights newest significant applications, and discusses future improvements, aiming to provide a practical guide for implementation of novel, effective, and efficient ALE experiments. Adaptive laboratory evolution (ALE) is a time-honored approach to obtain desired microbes in microbial engineering.Advanced strategies and tools facilitate and streamline ALE in growth coupling, genotypic diversification, phenotypic selection, and genotype–phenotype mapping.These strategies and tools have made considerable recent progress and have led to remarkable applications.
ISSN:0167-7799
1879-3096
DOI:10.1016/j.tibtech.2021.04.002