A dual-function chemical probe for detecting erasers of lysine lipoylation

Lysine lipoylation plays vital roles in cell metabolism and redox processes. For example, removal of lipoylation will decrease pyruvate dehydrogenase activity and affect the citric acid cycle. Despite the important functions of lysine lipoylation, the mechanisms for the addition and removal of this...

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Bibliographic Details
Published in:Frontiers of chemical science and engineering 2022, Vol.16 (1), p.121-127
Main Authors: Xie, Yusheng, Zhang, Jie, Yang, Liu, Chen, Qingxin, Hao, Quan, Zhang, Liang, Sun, Hongyan
Format: Article
Language:English
Subjects:
Antibodies
Chemistry
Chemistry and Materials Science
Citric acid
Communication
Industrial Chemistry/Chemical Engineering
Lysine
Mass spectrometry
Nanotechnology
Proteins
Purification
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Summary:Lysine lipoylation plays vital roles in cell metabolism and redox processes. For example, removal of lipoylation will decrease pyruvate dehydrogenase activity and affect the citric acid cycle. Despite the important functions of lysine lipoylation, the mechanisms for the addition and removal of this modification remain largely unexplored. Very few useful chemical tools are available to study the interactions of lysine lipoylation with its regulatory delipoylation proteins. For example, immunoaffinity purification-mass spectrometry is one of such tools, which highly relies on antibody efficiency and purification techniques. Single-step activity based fluorogenic probes developed by our groups and others is also an efficient method to study the deacylation activity. Affinitybased labeling probe using photo-cross-linker is a powerful platform to study the transient and dynamic interactions of peptide ligands with the interacting proteins. Herein, we have designed and synthesized a dual-function probe KTLlip for studying enzymatic delipoylation (eraser) activity and interaction of lysine lipoylation with the eraser at the same time. We show that KTLlip can be used as a useful tool to detect delipoylation as demonstrated by its ability to fluorescently label the eraser activity of recombinant Sirt2. We envision that the probe will help delineate the roles of delipoylation enzyme in biology.
ISSN:2095-0179
2095-0187
DOI:10.1007/s11705-021-2051-0