Perturbed ER homeostasis by IGF-IIRα promotes cardiac damage under stresses

• Published in: Molecular and cellular biochemistry 2022, Vol.477 (1), p.143-152
• Main Authors: Pandey, Sudhir, Kuo, Chia-Hua, Chen, William Shao-Tsu, Yeh, Yu-Lan, Kuo, Wei-Wen, Chen, Ray-Jade, Day, Cecilia Hsuan, Pai, Pei-Ying, Ho, Tsung-Jung, Huang, Chih-Yang
• Format: Article
• Language: English
• Subjects: Animals; Antibodies; Apoptosis; Autophagy; Biochemistry; Biomedical and Life Sciences; Cardiology; Cardiotoxicity; Caspase-12; Caspase-3; Cell Line; Congestive heart failure; Cytotoxins - adverse effects; Cytotoxins - pharmacology; Damage; Doxorubicin; Doxorubicin - adverse effects; Doxorubicin - pharmacology; Endoplasmic reticulum; Endoplasmic Reticulum - genetics; Endoplasmic Reticulum - metabolism; Endoplasmic Reticulum Stress; Heart failure; Homeostasis; Insulin-like growth factors; Kinases; Laboratory animals; Life Sciences; Medical Biochemistry; Myocardium - metabolism; Myocytes; Nutrients; Oncology; Oxidative stress; Perturbation; Proteins; Rats; Rats, Transgenic; Receptor, IGF Type 2 - genetics; Receptor, IGF Type 2 - metabolism; siRNA; Stress response; Transgenic animals
• ISSN: 0300-8177; 1573-4919
• DOI: 10.1007/s11010-021-04261-8

The heart is a very dynamic pumping organ working perpetually to maintain a constant blood supply to the whole body to transport oxygen and nutrients. Unfortunately, it is also subjected to various stresses based on physiological or pathological conditions, particularly more vulnerable to damages caused by oxidative stress. In this study, we investigate the molecular mechanism and contribution of IGF-IIRα in endoplasmic reticulum stress induction in the heart under doxorubicin-induced cardiotoxicity. Using in vitro H9c2 cells, in vivo transgenic rat cardiac tissues, siRNAs against CHOP, chemical ER chaperone PBA, and western blot experiments, we found that IGF-IIRα overexpression enhanced ER stress markers ATF4, ATF6, IRE1α, and PERK which were further aggravated by DOX treatment. This was accompanied by a significant perturbation in stress-associated MAPKs such as p38 and JNK. Interestingly, PARKIN, a stress responsive cellular protective mediator was significantly downregulated by IGF-IIRα concomitant with decreased expression of ER chaperone GRP78. Furthermore, ER stress-associated pro-apoptotic factor CHOP was increased considerably in a dose-dependent manner followed by elevated c-caspase-12 and c-caspase-3 activities. Conversely, treatment of H9c2 cells with chemical ER chaperone PBA or siRNA against CHOP abolished the IGF-IIRα-induced ER stress responses. Altogether, these findings suggested that IGF-IIRα contributes to ER stress induction and inhibits cellular stress coping proteins while increasing pro-apoptotic factors feeding into a cardio myocyte damage program that eventually paves the way to heart failure.