Detection of gamma-irradiated red peppers using a combination of 5,6-dihydrothymidine and thymidine as irradiation indicator

A method for determining the irradiation histories of plant-based food items was developed. The developed method was based on that used for determining the irradiation history of animal-based food items. The liquid chromatography–tandem mass spectrometry (LC-MS/MS) technique was used to analyze the...

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Bibliographic Details
Published in:Radiation physics and chemistry (Oxford, England : 1993) England : 1993), 2022-02, Vol.191, p.109849, Article 109849
Main Authors: Fukui, Naoki, Fujiwara, Takuya, Furuta, Masakazu, Takatori, Satoshi
Format: Article
Language:English
Subjects:
5,6-Dihydrothymidine
Cellulase
DNA extraction
Dosage
Food
Food irradiation history
Irradiation
Irradiation indicator
Liquid chromatography
Mass spectrometry
Peppers
Plant-based foods
Proteinase
Red pepper
Solid phases
Thymidine
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Summary:A method for determining the irradiation histories of plant-based food items was developed. The developed method was based on that used for determining the irradiation history of animal-based food items. The liquid chromatography–tandem mass spectrometry (LC-MS/MS) technique was used to analyze the total amount of (5S)- and (5R)-5,6-dihydrothymidine (sum of (5S)- and (5R)-DHdThd; total DHdThd), which are radiolytic products of thymidine (dThd). Red peppers were used as a model for plant-based food items. Powdered or homogenized red peppers were processed using lysis buffer-containing proteinase K, RNase A, and cellulase to obtain crude DNA. The crude DNA was further purified using a solid-phase extraction column. The DNA was enzymatically digested to produce nucleosides. Following this, the concentration of total DHdThd to dThd ratio (total DHdThd/dThd) was analyzed as an irradiation indicator using the LC-MS/MS technique. The total DHdThd/dThd in red peppers was increased in a dose-dependent manner. The presence of trace amounts of total DHdThd/dThd (
ISSN:0969-806X
1879-0895
DOI:10.1016/j.radphyschem.2021.109849