Polymorphisms and associations of the RACK1 genes with antibody response to Newcastle disease in KUB chickens

• Main Authors: Manzila, Ifa, Lestari, Puji, Sartika, Tike, Priyatno, Tri Puji, Indriani, Risa, Nugroho, Kristianto, Terryana, Rerenstradika Tizar
• Format: Conference Proceeding
• Language: English
• Subjects: Antibodies; Apoptosis; Biological activity; Kinases; Nucleotides; Polymorphism; Poultry
• ISSN: 0094-243X; 1551-7616
• DOI: 10.1063/5.0075622

Receptor for Activated C Kinase 1 (RACK1) is a gene involved in many biological processes, such as proliferation, cell migration, apoptosis, and innate immune responses. This study aimed to identify RACK1 gene polymorphisms and their associations with antibody response against Newcastle disease (ND) in Kampung Unggul Baru (KUB) chicken. Fifty KUB chickens were used in this study. Polymorphism of RACK1 gene was identified by PCR analysis and sequencing methods, while ND antibody response was detected by HI test. The results showed that antibody responses were grouped into low (12 of chickens) and high (38 of chickens) categories. Sequencing analyses of RACK1 gene determined double peaks at nucleotide position 1986 (SNP-1986) and 1993 (SNP-1993) indicating heterozygous genotype and a single peak as homozygous genotype. Both SNP-1986 and SNP-1993 were located in intron 4 of RACK1 gene. Association analyses showed that two intronic RACK1 polymorphisms were significantly associated with antibody response in KUB chickens to ND. These SNPs can be used as potential SNP markers for antibody response as parameters for ND resistance in KUB chicken.