Ultrasound-assisted Emulsified Microextraction Followed by High Performance Liquid Chromatography for Permethrin

Ultrasound assisted emulsification microextraction (UAEME) as a new sample preparation method was optimized for the determination of permethrin in urine samples. Also, deep eutectic solvent was used as the extracting solvent instead of organic solvents in this method. In order to determine the optim...

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Bibliographic Details
Published in:Journal of Siberian Federal University. Chemistry 2021-01, Vol.14 (4), p.447-456
Main Authors: Pourhossein, Mehran, Golaghaei, Alireza, Khaghani, Ramin, Paknezhad, Babak
Format: Article
Language:English
Subjects:
Chromatography
Emulsification
Food
High performance liquid chromatography
Hydrogen bonds
Methods
Optimization
Pesticides
Solvents
Ultrasonic imaging
Urine
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Summary:Ultrasound assisted emulsification microextraction (UAEME) as a new sample preparation method was optimized for the determination of permethrin in urine samples. Also, deep eutectic solvent was used as the extracting solvent instead of organic solvents in this method. In order to determine the optimal values, six main parameters were studied in different levels. Totally, 105 runs were performed using the one-variable-at-a-time method followed by high-performance liquid chromatography with a UV detector. Under the optimum conditions, the calibration curve for permethrin was linear in the concentration range of 5 to 500 μgL‑1 for urine samples. The accuracy and reproducibility of the introduced method were determined using the relative recovery (RR%) and relative standard deviation (RSD%) tests on the fortified urine samples. RR% and RSD% were found to be 96.3–101.7 % and 3.2–7.6 %, respectively. The limit of quantification and also the limit of detection were obtained 5 and 1 μg/L, respectively. In the present study, the DES-UA-EME technique was successfully developed for the extraction of permethrin from urine samples and subsequent quantification by high-performance liquid chromatography.In comparison to the other sample preparation methods, the proposed technique has the advantages of shorter extraction time, simplicity, and applicability in laboratories with less equipment
ISSN:1998-2836
2313-6049
DOI:10.17516/1998-2836-0253