Physical adsorption used to the immobilization of Burkholderia cepacia lipase into powder polymeric supports

• Published in: Journal of thermal analysis and calorimetry 2022-02, Vol.147 (4), p.3071-3081
• Main Authors: Corrêa, Wellington Moreira, Bortolozo, Ausdinir Danilo, Osório, Wislei Riuper, da Silva Padilha, Giovana
• Format: Article
• Language: English
• Subjects: Activation energy; Adsorption; Analysis; Analytical Chemistry; Chemistry; Chemistry and Materials Science; Copolymers; Divinylbenzene; Enthalpy; Immobilization; Inorganic Chemistry; Lipase; Measurement Science and Instrumentation; Morphology; Optical microscopy; Physical Chemistry; Polyhydroxybutyrate; Polymer Sciences; Polymers; Powders
• ISSN: 1388-6150; 1588-2926
• DOI: 10.1007/s10973-021-10714-5

This study aims to combine distinctive supports with a feasible, easy and cleaning method to lipase immobilization. Commercial lipase from Burkholderia cepacia is physically adsorbed using polymeric powders such as polyhydroxybutyrate (PHB), polyhydroxybutyrate-co-hydroxyvalerate (PHBV) and copolymer styrene-divinylbenzene (St-DVB). It is found that the immobilization on PHB has a high amount of the lipase per support mass (1065 U g −1 ). The activation energy also shows a value higher than the lipase immobilized onto both PHBV (348 U g −1 ) and St-DVB (355 U g −1 ). FTIR, TG, DSC and XRD techniques are used to polymer powders and immobilized lipase characterization. Morphological arrays, loss masses, enthalpy values and crystallinity degrees are evaluated. In order to analyze the morphological aspects of the raw polymers after alcohol treatment (before immobilization) and the immobilized lipase, the optical microscopy analysis is used. The immobilized Burkholderia cepacia lipase provides distinctive behavior in the polymer powders.