Statistical optimization of medium components for biosurfactant production by Pseudomonas guguanensis D30

• Published in: Preparative biochemistry & biotechnology 2022, Vol.52 (2), p.171-180
• Main Authors: Pardhi, Dimple S., Panchal, Rakeshkumar R., Raval, Vikram H., Rajput, Kiransinh N.
• Format: Article
• Language: English
• Subjects: Acetic acid; Calcium chloride; Central composite design; Chloroform; Culture Media; Design of experiments; Design optimization; Emulsification; emulsification index; Ethyl acetate; Experimental design; Fourier analysis; Fourier transforms; Indicators and Reagents - chemistry; Infrared spectroscopy; lipopeptide; Methanol; Micelles; mineral oil; Mineral oils; Peptones; Plackett-Burman design; Pseudomonas; Pseudomonas - metabolism; Pseudomonas guguanensis D30; Regression analysis; Statistical analysis; Surface Tension; Surface-Active Agents - metabolism; Surfactants; Yeast; Yeasts
• ISSN: 1082-6068; 1532-2297
• DOI: 10.1080/10826068.2021.1922919

Biosurfactant production by Pseudomonas guguanensis D30 was reported using mineral oil in submerged condition. Twelve medium components were tested at two levels by Plackett-Burman design, among them, mineral oil, yeast extract, peptone, MgSO 4 , and CaCl 2 found significant on the basis of emulsification index. These five significant components were further optimized through central composite design (CCD). The experimental design was successfully used for regression analysis and the significant model suggested the solution of 10% (v/v) mineral oil, 3.0 g/L (w/v) yeast extract and 0.2 g/L (w/v) peptone for 13.14 g/L predicted biosurfactant production. We kept the suggested concentrations of medium components and got 13.34 ± 0.08 g/L biosurfactant production, which is almost double the conventional one-factor-at-a-time production (7.126 ± 0.12 g/L). It reduced the surface tension of the medium up to 28 ± 1.2 mN/m. We found ethyl acetate a suitable solvent for biosurfactant extraction amongst methanol, chloroform, and methanol:chloroform. The partially purified biosurfactant was chemically characterized as lipopeptide by Fourier transform infrared spectroscopy (FT-IR).