Evaluation of the binding effect and cytotoxicity assay of 2‐Ethyl‐5‐(4‐methylphenyl) pyramido pyrazole ophthalazine trione on calf thymus DNA: spectroscopic, calorimetric, and molecular dynamics approaches

• Published in: Luminescence (Chichester, England) England), 2022-02, Vol.37 (2), p.310-322
• Main Authors: Marjani, Narges, Dareini, Maryam, Asadzade‐Lotfabad, Maryam, Pejhan, Mahtab, Mokaberi, Parisa, Amiri‐Tehranizadeh, Zeinab, Saberi, Mohammad Reza, Chamani, Jamshidkhan
• Format: Article
• Language: English
• Subjects: Analytical methods; Binding; Calf thymus; Chemical kinetics; Circular Dichroism; ct DNA–H1 complex; Cytotoxicity; Deoxyribonucleic acid; Dichroism; DNA; DNA - genetics; DNA structure; Dynamics; Enthalpy; Entropy; Fluorescence; Heat measurement; Histone H1; Histones; Hydrogen bonding; intercalative; Molecular Docking Simulation; Molecular dynamics; Molecular Dynamics Simulation; Pharmacokinetics; Pyrazole; Pyrazoles; Simulation; Spectrometry, Fluorescence; Spectrophotometry, Ultraviolet; Spectroscopic techniques; Spectroscopy; Spectrum analysis; Structural stability; Ternary systems; Thermodynamics; Thymus gland; Toxicity; Van der Waals forces; Viscosity
• ISSN: 1522-7235; 1522-7243
• DOI: 10.1002/bio.4173

With advances in new drug therapies, it is essential to understand the interactions between drugs and target molecules. In this study, we applied multiple spectroscopic techniques including absorbance, fluorescence, circular dichroism spectroscopy, viscosity, thermal melting, calorimetric, and molecular dynamics (MD) simulation to study the interaction between 2‐Ethyl‐5‐(4‐methylphenyl) pyramido pyrazole ophthalazine trione (PPF) and calf thymus DNA (ct DNA) in the absence or presence of histone H1. PPF exhibits a high binding affinity towards ct DNA in binary and ternary systems. In addition, the result for the binding constant was observed within the range 104 M−1 achieved through fluorescence quenching data, while the values for enthalpy and entropy changes for ct DNA–PPF and (ct DNA–H1) PPF complexes were measured to be −72.54 kJ.mol−1, −161.14 J.mol−1 K−1, −85.34 kJ.mol−1, and −19.023 J.mol−1 K−1, respectively. Furthermore, in accordance with circular dichroism spectra, the inducement of ct DNA structural changes was observed during binding of PPF and H1 in binary and ternary system forms. The essential roles of hydrogen bonding and van der Waals forces throughout the interaction were suggested using thermodynamic parameters. According to the obtained data, the interaction mode of ct DNA–PPF and (ct DNA–H1) PPF complexes was intercalation binding. Suggested by the MD simulation study, the ct DNA–H1 complex caused a reduction in the stability of the DNA structure in the presence or absence of ligand, which demonstrated that PPF as an intercalating agent can further distort the structure. The information achieved from this study will be very helpful in understanding the effects of PPF on the conformational state of ct DNA in the absence or presence of the H1 molecule, which seems to be quite significant for clarifying the mechanisms of action and its pharmacokinetics. The interaction between ct DNA–PPF in the absence or presence of H1 expresses the fact that PPF as an intercalating agent can further distort the structure.