Hydroxyapatite‐hybridized double‐network hydrogel surface enhances differentiation of bone marrow‐derived mesenchymal stem cells to osteogenic cells

Recently, we have developed a hydroxyapatite (HAp)‐hybridized double‐network (DN) hydrogel (HAp/DN gel), which can robustly bond to the bone tissue in the living body. The purpose of this study is to clarify whether the HAp/DN gel surface can differentiate the bone marrow‐derived mesenchymal stem ce...

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Bibliographic Details
Published in:Journal of biomedical materials research. Part A 2022-04, Vol.110 (4), p.747-760
Main Authors: Kaibara, Takuma, Wang, Lei, Tsuda, Masumi, Nonoyama, Takayuki, Kurokawa, Takayuki, Iwasaki, Norimasa, Gong, Jian Ping, Tanaka, Shinya, Yasuda, Kazunori
Format: Article
Language:English
Subjects:
Alkaline phosphatase
Animals
Biomarkers
Biomedical materials
Bone marrow
Bone Marrow - metabolism
Bone Marrow Cells - metabolism
Cbfa-1 protein
Cell differentiation
Cell Differentiation - genetics
Cells, Cultured
double‐network hydrogel
Durapatite - chemistry
Flow cytometry
Gene expression
Hydrogels
Hydrogels - metabolism
Hydrogels - pharmacology
Hydroxyapatite
hydroxyapatite‐hybridized hydrogel
in vitro effect
mesenchymal stem cell
Mesenchymal Stem Cells
Osteogenesis - genetics
osteogenic differentiation
Plugs
Polymerase chain reaction
Polystyrene
Polystyrene resins
Rabbits
Stem cells
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Summary:Recently, we have developed a hydroxyapatite (HAp)‐hybridized double‐network (DN) hydrogel (HAp/DN gel), which can robustly bond to the bone tissue in the living body. The purpose of this study is to clarify whether the HAp/DN gel surface can differentiate the bone marrow‐derived mesenchymal stem cells (MSCs) to osteogenic cells. We used the MSCs which were harvested from the rabbit bone marrow and cultured on the polystyrene (PS) dish using the autogenous serum‐supplemented medium. First, we confirmed the properties of MSCs by evaluating colony forming unit capacity, expression of MSC markers using flow cytometry, and multidifferential capacity. Secondly, polymerase chain reaction analysis demonstrated that the HAp/DN gel surface significantly enhanced mRNA expression of the eight osteogenic markers (TGF‐β1, BMP‐2, Runx2, Col‐1, ALP, OPN, BSP, and OCN) in the cultured MSCs at 7 days than the PS surfaces (p 
ISSN:1549-3296
1552-4965
DOI:10.1002/jbm.a.37324