Automated spectrophotometric platform for the quantification of multiple nucleic acid samples

A novel automated spectrophotometric platform was developed to upgrade a conventional prototype instrument platform for commercial use. A pulsed xenon flash lamp was chosen as the light source to improve the light output and a microsampling system was employed to precisely and rapidly aspirate micro...

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Bibliographic Details
Published in:Instrumentation science & technology 2021-11, Vol.50 (3), p.334-350
Main Authors: Kang, Hoon, Park, Sang-Ryoul, Kim, Ju Hwang, Park, In-Yong, Yoo, Hee-Bong, Yang, Inchul, Jung, Haewon
Format: Article
Language:English
Subjects:
Automated spectrophotometric platform
Automation
Biopsy
Deoxyribonucleic acid
DNA
DNA sequencing
Electric components
Flash lamps
Forensic science
light absorbance measurement
Light sources
micro-volume spectrophotometer
Next-generation sequencing
nucleic acid quantification
Nucleic acids
Quality control
Spectrophotometry
Three axis
Xenon
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Summary:A novel automated spectrophotometric platform was developed to upgrade a conventional prototype instrument platform for commercial use. A pulsed xenon flash lamp was chosen as the light source to improve the light output and a microsampling system was employed to precisely and rapidly aspirate micro-volume sample solutions. In addition, a three-axis positioning system was devised to increase the measurement capacity. All electrical components of the developed platform were linked and controlled with an integrated system. To verify the measurement performance and automated operation, a series of experiments were carried out. Double-stranded DNA samples at seven concentrations were automatically quantified. The concentrations were estimated with high linearity (R 2 ≒ 0.9998), repeatability (relative standard deviation ≤10.3%), and accuracy (>87.5%) across the concentration range from 0.25 to 10 ng/μL. In addition, this platform can be used in many different ways by changing a few parts or a sub-system and will be most useful in laboratories that handle a large number of samples. It is expected that the developed platform will be utilized for measuring circulating cell-free DNA and for quality control of next-generation sequencing, forensic medicine, noninvasive prenatal tests, and liquid biopsies.
ISSN:1073-9149
1525-6030
DOI:10.1080/10739149.2021.2001000