Utilization of Ocimum basilicum extracts for zinc oxide nanoparticles synthesis and their antibacterial activity after a novel combination with phage

•Green synthesis of Zinc oxide nanoparticles (ZnO-NPs) was done by Ocimum basilicum.•Various techniques were conducted to characterize the biosynthesized ZnO-NPs.•The ZnO-NPs were safe for phage in the concentration that kills the Salmonella.•The ZnO-NPs had shown a synergetic antibacterial activity...

Full description

Saved in:
Bibliographic Details
Published in:Materials letters 2022-02, Vol.309, p.131344, Article 131344
Main Authors: Abdelsattar, Abdallah S., Farouk, Warda M., Mohamed Gouda, Shrouk, Safwat, Anan, Hakim, Toka A., El-Shibiny, Ayman
Format: Article
Language:English
Subjects:
Antibacterial agent
Bacteria
Biological materials
Biomaterials
Electron micrographs
Green synthesis
Irregular particles
Materials science
Nanoparticles
Phage therapy
Phages
Salmonella
Synergetic effect
Zinc oxide
Zinc oxides
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:•Green synthesis of Zinc oxide nanoparticles (ZnO-NPs) was done by Ocimum basilicum.•Various techniques were conducted to characterize the biosynthesized ZnO-NPs.•The ZnO-NPs were safe for phage in the concentration that kills the Salmonella.•The ZnO-NPs had shown a synergetic antibacterial activity when combined with phage.•The ZnO-NPs showed biofilm inhibition that can be formed by Staphylococcusbacteria. The current study illustrates the synergetic effect of both environment-friendly green synthesized zinc oxide nanoparticles (ZnO-NPs) of Ocimum basilicum extract using the bio-reduction process and bacteriophages (phage) to control pathogenic bacteria. Ocimum basilicum has various phytochemical compounds that were responsible for the formation of ZnO-NPs. In addition, the phage ZCSE6, as a biological material, has a bacteriolytic activity against pathogenic bacteria. The spectrum of UV–Vis indicated a corresponding ZnO-NPs peak at 370 nm. Transmission electron micrographs (TEM) showed irregular particles in shape with 10–25 nm in size. Furthermore, the ZnO-NPs displayed biofilm deformation that can be formed by Staphylococcussciuri(S. sciuri). Moreover, it has a synergic antibacterial activity against Salmonella enterica (S. enterica) when combined with phage ZCSE6. This study reveals the antibacterial effect of using metallic and biological materials and their possible applications in the medical field.
ISSN:0167-577X
1873-4979
DOI:10.1016/j.matlet.2021.131344