The Influence of Pantogam and Atomoxetine on Attention Stability and Distribution of Dopamine D2 and GABAB Receptors in the Attention Deficit Mouse Model

• Published in: Biochemistry (Moscow). Supplement. Series B, Biomedical chemistry Biomedical chemistry, 2022-03, Vol.16 (1), p.30-37
• Main Authors: Kovalev, G. I., Sukhorukova, N. A., Vasileva, E. V., Kondrakhin, E. A., Salimov, R. M.
• Format: Article
• Language: English
• Subjects: Bioorganic Chemistry; Chemistry; Chemistry and Materials Science; Dopamine; Dopamine D2 receptors; Exploratory behavior; Locomotor activity; Medicinal Chemistry; Prefrontal cortex; Receptor density; Subpopulations; γ-Aminobutyric acid B receptors
• ISSN: 1990-7508; 1990-7516
• DOI: 10.1134/S1990750822010048

— The closed enriched cross maze test was employed as a novel experimental model of the attention deficit disorder (ADD) for evaluation of the behavioral and neurochemical effects of the nootropic drug pantogam (100 mg/kg, intraperitoneally) and atomoxetine hydrochloride (3 mg/kg, intraperitoneally) administered subchronically to CD-1 outbred mice. Two subpopulations of rodents spontaneously diverging in attention to enriched compartments (ED-Low and ED-High), were estimated on the basis of time spent by the mice in the empty or enriched compartments. The ED-Low and ED-High mice did not differ in parameters associated with anxiety, exploratory efficacy, and locomotor activity. Subchronic administration of both drugs in selected doses produced a corrective effect on animal behavior manifested as selective increase in the ED-ratio values in the ED-Low subpopulation ( p < 0.05). The radioligand binding assays revealed differences in the distribution of dopamine D 2 and GABA B receptors ( B max ) in prefrontal cortex of control ED-Low and ED-High mice. In prefrontal cortex of ED-Low mice treatment with atomoxetine produced a decrease in the B max values of D 2 receptors by 14%, while pantogam decreased the B max values of D 2 receptors by 22% ( p