Natural sea salt based polyhydroxyalkanoate production by wild Halomonas hydrothermalis strain

• Published in: Fuel (Guildford) 2022-03, Vol.311, p.122593, Article 122593
• Main Authors: Dubey, Sonam, Mishra, Sandhya
• Format: Article
• Language: English
• Subjects: Analytical studies; Biodegradability; Biodegradation; Biopolymers; Commercialization; Dry Sea Mix (DSM); Feasibility studies; Glycerol; Halomonas; Mechanical properties; Peptones; Polyhydroxyalkanoates; Polyhydroxyalkanoic acid; Polyhydroxybutyrate; Salts; Statistical approach; Substrates; Sustainability
• ISSN: 0016-2361; 1873-7153
• DOI: 10.1016/j.fuel.2021.122593

[Display omitted] •A resolution towards cleaner production from waste to value added products.•An efficient form of natural sea salt (CSMCRI’s Dry Sea Mix) as media component.•Faster metabolism rate of wild bacteria grown in natural substrates was observed. Polyhydroxyalkanoates (PHAs) are emerging potential biopolymers replacing the hazardous petroleum based plastics with several applications and biodegradability. The major threat towards its commercialization is the substrate cost which could be replaced with crude byproducts or natural substrates for its sustainability at industrial scale. A wild marine bacterial strain Halomonas hydrothermalis (MTCC 5445) was used as a source for PHA accumulation. An effort has been made to use a sea salt formulation which is a natural substrate used both as growth and production media in our current study. To check the feasibility of this sea salt formulation, it was used in combination with glycerol and peptone as carbon and nitrogen source respectively in production media. A shake flask experiment with 20 run was set up with maximum PHA productivity of 2.61 g/L in 3% Glycerol, 3% DSM and 0.55% peptone set. The extracted PHA properties were checked through various analytical tools and compared with standard PHB indicating similarity in both structural and mechanical properties.