Phytotoxicity in aqueous methanolic extracts of rice against junglerice and total activities of identified phytotoxic compounds

• Published in: Annals of applied biology 2022-03, Vol.180 (2), p.196-210
• Main Authors: Ho, Thi L., Nguyen, Van L., Phan, Linh K., Nguyen, Cuong T., Nguyen, Trang H. D., Van, Vang L., Reid, Smeda J.
• Format: Article
• Language: English
• Subjects: Acids; Allelochemicals; allelopathy; Benzoic acid; Cinnamic acid; Coumaric acid; Coumarin; Crop production; Cultivars; Echinochloa colona; Echinochloa colona L; Invasive plants; Oryza sativa; Oryza sativa L. cv. OM; Phytotoxicity; Rice; Salicylic acid; Seedlings; total activity; Vanillic acid
• ISSN: 0003-4746; 1744-7348
• DOI: 10.1111/aab.12746

This study examined phytotoxic activities of several Vietnam localised OM rice (Oryza sativa L. OM) cultivars on junglerice (Echinochloa colona (L.) Link), an invasive weed threatening rice production, and identified allelochemicals and their activities for OM 4498, the most allelopathic cultivar. The aqueous MeOH extracts of all rice cultivars inhibited root and shoot growth of junglerice at a concentration as low as 0.01 g ml−1. Inhibitory effects were greatest in OM 380, OM 2395 and OM 4498, with the average effective doses (EDs) of extracts required for 90% inhibition (ED90) on junglerice as low as 0.981, 0.632 and 0.702 g ml−1, respectively. The phytotoxic substances salicylic acid, vanillic acid, p‐coumaric acid, 2,4‐dimethoxybenzoic acid, benzoic acid, p‐hydrobenzoic acid, trans‐cinnamic acid and coumarin were identified in OM 4498 at concentrations ranging from 0.066 to 11.412 mg in 100 g fresh weight of rice tissue. Two of these substances, 2,4‐dimethoxybenzoic acid and coumarin, have never previously been identified in rice. Compared to other compounds, p‐coumaric acid may have the greatest weed‐suppressant potential on junglerice, with the average ED50 being 0.28 mM. The total activities of salicylic acid, vanillic acid, p‐coumaric acid, trans‐cinnamic acid and 2,4‐dimethoxybenzoic acid in an OM 4498 rice plant on junglerice seedlings were 59.60, 0.78, 3.47, 2.52 and 0.61 mM/mM, respectively. The results clearly suggest that OM 4498 rice extract is an excellent source for allelochemicals against junglerice, and this rice cultivar should be considered in rice production areas invaded by junglerice. Rice (Oryza sativa L. OM 4498) releases allelochemicals which strongly inhibit the growth and development of junglerice (Echinochloa colona L.), one of invasive weeds threatening rice production