Effect of Copper Nanoparticles and Ions on Epididymis and Spermatozoa Viability of Chinese Soft-Shelled Turtles Pelodiscus sinensis

Copper nanoparticles (CuNPs) have been widely used in various industrial and commercial applications, which become a potential threat to aquatic organisms. Nevertheless, their potential toxicity to the epididymis and sperm remains little known. In this study, we evaluated the effect of CuNPs and cop...

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Bibliographic Details
Published in:Coatings (Basel) 2022-02, Vol.12 (2), p.110
Main Authors: Yang, Li, Wei, Yating, Gao, Shuai, Wang, Qifei, Chen, Jiaqi, Tang, Boping, Bian, Xunguang
Format: Article
Language:English
Subjects:
Antioxidants
Biomarkers
Connective tissues
Copper
Copper sulfate
Crustaceans
Cytokines
Efficiency
Enzyme activity
Enzymes
Epithelium
Experiments
Gene expression
Microscopy
Motility
Nanomaterials
Nanoparticles
Organisms
Physiology
Pollutants
Reptiles & amphibians
Sperm
Spermatozoa
Toxicity
Trout
Turtles
Viability
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Summary:Copper nanoparticles (CuNPs) have been widely used in various industrial and commercial applications, which become a potential threat to aquatic organisms. Nevertheless, their potential toxicity to the epididymis and sperm remains little known. In this study, we evaluated the effect of CuNPs and copper ions (CuSO4) on the spermatozoa viability, epididymal structure, antioxidant enzyme activity, and inflammatory cytokines in cauda epididymis of the Chinese soft-shelled turtle. Results showed that the spermatozoa viability of Chinese soft-shelled turtles decreased significantly with an increase in CuNPs or Cu ions concentrations. The epithelial cells of the epididymal duct of the Chinese soft-shelled turtles with the treatment of 5 mg kg−1 CuNPs were slightly swollen, and the connective tissue between the epididymal ducts was loose. The epithelial structure of the epididymal tube was severely damaged with an increase in Cu ion concentrations. Compared to the control, the antioxidative enzymes activities and the expression of IL-1β, TNF-α, and IL-6 mRNA in the epididymis significantly increased with the treatment of CuNPs or CuSO4. The present study revealed that Cu ions exert more harmful effect on the epididymis and spermatozoa viability of Chinese soft-shelled turtles than copper nanoparticles.
ISSN:2079-6412
2079-6412
DOI:10.3390/coatings12020110