Expression of immune-related proteins and their association with neuropeptides in adolescent patients with anorexia nervosa

• Published in: Neuropeptides (Edinburgh) 2022-02, Vol.91, p.102214, Article 102214
• Main Authors: Tyszkiewicz-Nwafor, Marta, Jowik, Katarzyna, Paszynska, Elzbieta, Dutkiewicz, Agata, Słopien, Agnieszka, Dmitrzak-Weglarz, Monika
• Format: Article
• Language: English
• Subjects: Adipocytokines; Adiponectin; Adiponectin - blood; Adolescent; Anorexia; Anorexia nervosa; Anorexia Nervosa - blood; Chemokines; Cytokines; Cytokines - blood; Eating disorders; Fasting - blood; Female; Fibroblast growth factor 2; Gelatinase B; Humans; Inflammation; Interleukin 1; Interleukin 6; Interleukin 8; Leptin; Leptin - blood; Leptin receptors; Neuropeptides; Neurotransmitters; Neutrophil collagenase; Patients; Proteases; Proteins; Resistin; Resistin - blood
• ISSN: 0143-4179; 1532-2785
• DOI: 10.1016/j.npep.2021.102214

Anorexia nervosa (AN) is a metabo-psychiatric disorder where alterations of cytokines, neuropeptides, neurotransmitters, and the interactions between these factors can play an important role. Thus, the primary goal of the presented study was a cross-sectional analysis of immune-related proteins in patients with AN. Moreover, the correlations between these molecules and selected neuropeptides were studied. Twenty-five adolescent inpatients girls in the acute stage of a restrictive type of AN were enrolled in the study within the first year of the disease. Additionally, thirty similar in age and height controls (CG) were also assessed. The levels of 24 immune-related proteins, including cytokines, chemokines, and proteases, were measured. Moreover, selected adipocytokines, gastrointestinal hormones, and centrally produced neuropeptides levels were determined. Finally, the correlations between these molecules were analyzed. The fasting levels of CXCL1, CXCL9, FGF2, GrB, IL1, IL6, IL8, MMP8, MMP9, CTSS were statistically lower in AN than in the CG. The concentrations of many immune-related proteins remain unchanged despite their metabolic and mental condition. Moreover, significant correlations were found between leptin and CXCL1, CXCL9, GrB, IL1, IL6, and MMP8. Leptin receptors were correlated with GrB, while resistin was associated with MMP9. Our findings suggest that the initial stage of restrictive AN among adolescents within the first year of the disease is not connected with a pro-inflammatory state. Some immune-related protein changes may be associated with altered neuropeptides, primarily leptin, its receptors, and resistin. Future research should clarify which changes are primary and secondary to weight loss and whether these changes normalize with increasing weight. This would aid in understanding the complex etiopathogenesis of AN and in the search for new methods of treatment. •IL-1, IL-6, IL-8 are decreased in an adolescent patient with anorexia nervosa.•CXCL1, CXCL9 are decreased in an adolescent patient with anorexia nervosa.•MMP, MMP9, CTSS and GrB are decreased in an adolescent patient with anorexia nervosa.•Leptin is related to altered immune-related proteins in the anorexia nervosa.•Resistin is related to altered immune-related proteins in the anorexia nervosa.