Development of QTF-based mass-sensitive immunosensor for phenylketonuria diagnosis

In this study, a biosensor was prepared with bilayer plasma polymer films coated on a quartz tuning fork (QTF) for simple, selective, and sensitive determination of L -phenylalanine. Two-step surface modification of QTFs’ prongs was performed through hexane and ethylenediamine plasma, respectively....

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Bibliographic Details
Published in:Applied physics. A, Materials science & processing Materials science & processing, 2022-04, Vol.128 (4), Article 277
Main Authors: Kaleli-Can, Gizem, Özgüzar, Hatice Ferda, Mutlu, Mehmet
Format: Article
Language:English
Subjects:
Antibodies
Applied physics
Biosensors
Characterization and Evaluation of Materials
Condensed Matter Physics
Ethylenediamine
Hexanes
Immunosensors
Machines
Manufacturing
Materials science
Nanotechnology
Optical and Electronic Materials
Phenylalanine
Physics
Physics and Astronomy
Polymer films
Processes
Surfaces and Interfaces
Thin Films
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Summary:In this study, a biosensor was prepared with bilayer plasma polymer films coated on a quartz tuning fork (QTF) for simple, selective, and sensitive determination of L -phenylalanine. Two-step surface modification of QTFs’ prongs was performed through hexane and ethylenediamine plasma, respectively. Functionalized QTFs’ surfaces were treated with glutaraldehyde at different time intervals to activate prong surfaces for antibody immobilization. Then, anti-phenylalanine was attached to the activated prongs of QTFs. The linear range for phenylalanine was found to be from 5 to 200 μM. The sensitivity of the developed QTF-based immunosensor was obtained 0.3809 Hz/μM. Graphical Abstract
ISSN:0947-8396
1432-0630
DOI:10.1007/s00339-022-05439-7