Localized surface plasmon-enhanced electrochemiluminescence biosensor for rapid, label-free, and single-step detection of broad-spectrum bacteria using urchin-like Au and Ag nanoparticles

• Published in: Sensors and actuators. B, Chemical Chemical, 2022-03, Vol.355, p.131120, Article 131120
• Main Authors: Han, Daobin, Li, Xinmin, Bian, Xintong, Wang, Jianmin, Kong, Liangsheng, Ding, Shijia, Yan, Yurong
• Format: Article
• Language: English
• Subjects: Ag nanoparticles; Bacteria; Bacterial analysis; Biosensors; Electrochemiluminescence; Electrochemiluminescence biosensor; Gold; Localized surface plasmon resonance; Nanoparticles; Nucleic acids; Silver; Target recognition; Urchin-like Au
• ISSN: 0925-4005; 1873-3077
• DOI: 10.1016/j.snb.2021.131120

In this study, localized surface plasmon-enhanced electrochemiluminescence (LSP-ECL) biosensor, a new ECL enhancement way for S2O82−/O2 system, was constructed based on urchin-like Au and Ag nanoparticles (AgNPs) for fast, one-step, and label-free detection of broad-spectrum bacteria. We found plasmonic metals with small diameters contributed to the ECL enhancement. Moreover, relying entirely on the physical puncture of urchin-like Au, the bacteria could be captured to the electrode surface without the need for target recognition agents. The single-step LSP-ECL biosensor exhibited high sensitivity in the range of 102–108 CFU/mL bacteria with the detection limit as low as 52 CFU/mL, meanwhile possessing a short detection time of 30 min. What’s more, the LSP-ECL biosensing strategy achieved detection of S.aureus present in plasma samples without involving bacterial culture and nucleic acid extraction. Considering the merits of label-free, short assay time, and one-step operation, the developed LSP-ECL strategy provides a versatile and culture-free platform for pathogenic bacteria detection. •The LSP-ECL biosensor was developed for rapid, label-free, and single-step bacterial detection.•The urchin-like Au effectively capture broad-spectrum bacteria without target recognition agents.•A new ECL enhancement way for S2O82−/O2 system was explored to analyze bacteria.•The strategy achieved label-free and single-step quantification of bacteria within 30 min.