Single‐Vesicle Electrochemistry Reveals Sex Difference in Vesicular Storage and Release of Catecholamine

Quantitative measurements of sex difference in vesicle chemistry (i.e., chemical storage and release) at the single‐vesicle level are essential to understand sex differences in cognitive behaviors; however, such measurements are very challenging to conventional analytical methods. By using single‐ve...

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Bibliographic Details
Published in:Angewandte Chemie 2022-03, Vol.134 (14), p.n/a
Main Authors: Yue, Qingwei, Wang, Kai, Guan, Ming, Zhao, Zhenwen, Li, Xianchan, Yu, Ping, Mao, Lanqun
Format: Article
Language:English
Subjects:
Analytical methods
Calcium channels
Calcium channels (voltage-gated)
Catecholamine
Catecholamines
Chemistry
Chromaffin cells
Cognitive ability
Dimorphism
Electrochemistry
Exocytosis
Gender aspects
Lipid composition
Lipids
Males
Neurotransmitter release
Neurotransmitters
Rodents
Sex Difference
Sex differences
Sexual behavior
Sexual dimorphism
Single-Vesicle Electrochemistry
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Summary:Quantitative measurements of sex difference in vesicle chemistry (i.e., chemical storage and release) at the single‐vesicle level are essential to understand sex differences in cognitive behaviors; however, such measurements are very challenging to conventional analytical methods. By using single‐vesicle electrochemistry, we find the duration of single exocytotic events of chromaffin cells prepared from male rats is statistically longer than that from female rats, leading to more neurotransmitter released in the male group. Further analysis reveals that a higher percentage of vesicles in the female group release part of the neurotransmitter, i.e., partial release, during exocytosis than that in male group. This sex dimorphism in neurotransmitter release in exocytosis might relate to the sex difference in the expression of voltage‐dependent calcium channels and membrane lipid composition. Our finding offers the first experimental evidence that sex dimorphism even exists in vesicle chemistry, providing a brand new viewpoint for understanding the sex dimorphism in exocytosis. Electrochemical quantitative measurements of vesicle chemistry (i.e., chemical storage and release) at the single‐vesicle level reveal a sex difference in transmitter release during exocytosis, which might relate to the sex difference of the expression level of voltage‐dependent calcium channels and membrane lipid composition. This research provides a new perspective to understand sex dimorphism in exocytosis.
ISSN:0044-8249
1521-3757
DOI:10.1002/ange.202117596