Avian metapneumovirus subtype B vaccination in commercial broiler chicks: heterologous protection and selected host transcription responses to subtype A or B challenge

Avian metapneumovirus (aMPV) causes respiratory disease and drops in egg production in chickens, and is routinely controlled by vaccination. However, the host's immune response to virulent challenge in vaccinated or unvaccinated broiler chickens is poorly characterized. We show that subtype B v...

Full description

Saved in:
Bibliographic Details
Published in:Avian pathology 2022-04, Vol.51 (2), p.181-196
Main Authors: Ball, Christopher, Manswr, Basim, Herrmann, Andreas, Lemiere, Stephane, Ganapathy, Kannan
Format: Article
Language:English
Subjects:
Animals
Antibodies
Antibodies, Viral
Avian metapneumovirus
Bone
broiler chicken
CD8 antigen
Cell-mediated immunity
Chickens
cross-protection
Defence mechanisms
Egg production
Gene expression
gene transcription
Homology
IL-1β
Immune response
Immune system
Immunity
Immunity, Cellular
Immunoglobulin A
Interleukin 18
Interleukin 6
Metapneumovirus - genetics
Paramyxoviridae Infections - prevention & control
Paramyxoviridae Infections - veterinary
Poultry
Poultry Diseases
Protection
Respiratory diseases
Respiratory disorders
Tissue
TLR3 protein
Toll-like receptors
Trachea
Transcription
Vaccination
Vaccination - veterinary
Viral infections
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Avian metapneumovirus (aMPV) causes respiratory disease and drops in egg production in chickens, and is routinely controlled by vaccination. However, the host's immune response to virulent challenge in vaccinated or unvaccinated broiler chickens is poorly characterized. We show that subtype B vaccination offers heterologous (subtype A challenge) and homologous (subtype B challenge) protection. Subtype B challenge caused significantly greater humoral antibody titres in vaccinated and unvaccinated chickens. In turbinate and lung tissues of unvaccinated-challenged chickens, IgA and IgY mRNA transcription was significantly up-regulated after subtype B challenge compared to subtype A. Cellular immunity (CD8-α and CD8-β) gene transcripts were significantly up-regulated during early and later stages of infection from subtype B or subtype A, respectively. Immune gene transcriptional responses (IL-1β, IL-6 and IL-18) were significantly up-regulated after challenge. Gene transcription results showed that mRNA expression levels of CD8-α, CD8-β, TLR3 and IL-6, particularly in turbinate and trachea tissues, are useful parameters to include in future aMPV vaccination-challenge studies.
ISSN:0307-9457
1465-3338
DOI:10.1080/03079457.2022.2036697