Nigella sativa flavonoids surface coated gold NPs (Au-NPs) enhancing antioxidant and anti-diabetic activity

• Published in: Process biochemistry (1991) 2022-03, Vol.114, p.193-202
• Main Authors: Veeramani, Subha, Narayanan, Arya P., Yuvaraj, Kousika, Sivaramakrishnan, Ramachandran, Pugazhendhi, Arivalagan, Rishivarathan, Ilangovan, Jose, Sujin P., Ilangovan, Rajangam
• Format: Article
• Language: English
• Subjects: Anthraquinone; Anthraquinones; Anticancer; Anticancer properties; Antidiabetic; Antioxidants; Biocompatibility; Coating; Diabetes; Diabetes mellitus; Flavonoids; Functional groups; Gold; Gold coatings; Gold NPs; Green synthesis; Nanoparticles; Nigella sativa; Phytochemicals; Reducing agents; Scavenging; Synthesis
• ISSN: 1359-5113; 1873-3298
• DOI: 10.1016/j.procbio.2021.01.004

[Display omitted] •Nigella sativa seed extract as an gold nanoparticles (AuNPs) surface coating material.•Nigella sativa seed extract efficiently produced the AuNPs.•Synthesized AuNPs showed high antioxidant and antidiabetic activity.•Greener synthesis of AuNPs has high anti-diabatic property. In the present investigation, gold NPs (Au-NPs) were synthesized via seed decoction of Nigella sativa, in which, the aqueous seed extract of Nigella sativa acted as a reducing agent as well as coated on Au-NPs surface. Herein, an attempt was made to develop a rapid and biocompatible method to synthesize gold NPs by phytofabrication for anti-diabetic applications. The Nigella sativa seed extract and the Au-NPs synthesized using Nigella sativa exhibited a good antioxidant property, which was determined by the 2, 2- Diphenylpicrylhydrazyl (DPPH) scavenging activity assay. These Au-NPs exhibited 78 % of anti- diabetic property, which was confirmed by α- amylase method. The synthesized nanoparticle Au-NPs size was confirmed by using SEM and TEM. The functional groups (thymoqninone) present in the seed extract as well as Au-NPs were analysed by FTIR. The major functional group i.e. thymoquinone was confirmed by the anthraquinone test and it is responsible for the antioxidant, anti-diabetic, and anticancer properties of the Au-NPs. The phytochemical based nanoparticle prepared in this work showed efficient anti-diabetic activities and it can be considered for the medical applications. In addition, phytochemical based nanoparticle synthesis is safe, eco-friendly, biocompatible, and cost effective.