Effect of Static and Dynamic Stretching on Corneal Fibroblast Cell

A strain gradient was created by punching a hole in the center of a stretched elastic polydimethylsiloxane membrane to determine the effect of different strains on cultured human keratocytes (HK). In this study, two stretching methods were used: continuous stretching and cyclic stretching. Continuou...

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Bibliographic Details
Published in:Processes 2022-03, Vol.10 (3), p.605
Main Authors: Dai, Zhi-Xuan, Shih, Po-Jen, Yen, Jia-Yush, Wang, I-Jong
Format: Article
Language:English
Subjects:
Cell death
Cell proliferation
Cell survival
Collagen
Cornea
Cyclic loads
Experiments
Fibroblasts
Image processing
Intraocular pressure
Polydimethylsiloxane
Pressure effects
Rubbing
Shear strain
Shear stress
Simulation
Software
Statistical analysis
Strain
Stress concentration
Stretching
Ultrasonic imaging
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Summary:A strain gradient was created by punching a hole in the center of a stretched elastic polydimethylsiloxane membrane to determine the effect of different strains on cultured human keratocytes (HK). In this study, two stretching methods were used: continuous stretching and cyclic stretching. Continuous stretching is relatively static, while acyclic stretching is relatively dynamic. These methods, respectively, represented the effects of high intraocular pressure and rubbing of the eyes on corneal cells. Image processing codes were developed to observe the effects of stress concentration, shear stress, continuous stretching, and cyclic stretching on HKs. The results demonstrate that stretching and shear stress are not conducive to the proliferation of corneal cells and instead cause cell death. A 10% strain had greater inhibitory effects than a 3% strain on cell proliferation. Cell survival rates for continuous stretching (static) were higher than those for cyclic stretching (dynamic). The stretching experiment revealed that cyclic stretching has a greater inhibitory effect on the growth and proliferation of corneal cells than continuous stretching. Accordingly, it shows that cyclic loading is more harmful than high intraocular pressure (static loading) to corneal cells.
ISSN:2227-9717
2227-9717
DOI:10.3390/pr10030605