Effects of probiotics on the severity of experimental acute pancreatitis

• Published in: European journal of clinical nutrition 2006-04, Vol.60 (4), p.464-468
• Main Authors: Muftuoglu, M.A.T, Isikgor, S, Tosun, S, Saglam, A
• Format: Article
• Language: English
• Subjects: acute course; animal models; Animals; Arginine; Arginine - pharmacology; Biological and medical sciences; Clinical Nutrition; diet; dietary supplements; disease severity; Edema; enteral feeding; Epidemiology; Evaluation; experimental diets; Experiments; Fibrosis; Gastroenterology. Liver. Pancreas. Abdomen; human diseases; human nutrition; Immunohistochemistry; Infiltration; Injection; Internal Medicine; intraperitoneal injection; Leukocytes; Leukocytes (mononuclear); Liver. Biliary tract. Portal circulation. Exocrine pancreas; Medical disorders; Medical sciences; Medicine; Medicine & Public Health; Metabolic Diseases; Necrosis; Nutrition; original-article; Other diseases. Semiology; Pancreas; Pancreas - drug effects; Pancreas - pathology; Pancreatitis; Pancreatitis, Acute Necrotizing - chemically induced; Pancreatitis, Acute Necrotizing - pathology; Pancreatitis, Acute Necrotizing - prevention & control; Probiotics; Public Health; Random Allocation; Rats; Rats, Wistar; Rodents; Severity of Illness Index; Treatment Outcome
• ISSN: 0954-3007; 1476-5640
• DOI: 10.1038/sj.ejcn.1602338

Objective: This study was designed to evaluate the effects of probiotics on the severity of experimental acute pancreatitis. Design: Experimental study. Setting: Experiments were done in a laboratory at Haydarpasa Numune Teaching and Research Hospital. Subjects: A total of 50 Wistar rats were randomly divided into five groups. Interventions: Group 1 was control group. Group 2 received an intraperitoneal injection of a 20% solution in 0.15 mol/l NaCL. Group 3 was injected NaCL and fed with probiotics. Acute pancreatitis was induced in rats by intrperitoneal injection of L-Arginine in groups 4 and 5. The rats in group 5 were treated with probiotics. The pancreas was removed for histologic examination. Evaluation of the pathologic changes was done by a new combined histopathologic grading scale. Results: The mean scores of fibrosis, acinar cell loss, oedema, parenchymal necrosis, mononuclear cells infiltration, polymorphonuclear leucocytes infiltration, ductal damage and atypical reactive regeneration in group 5 were significantly lower than group 4. Conclusions: We demonstrated that enteral feedings with added probiotics can reduce the severity of acute pancreatitis. Sponsorship: None.