Preparation and Properties of Complexes Based on Chitosan-Ag Nanocomposite and Cephalosporin Antibiotics

• Published in: Applied biochemistry and microbiology 2022-04, Vol.58 (2), p.136-142
• Main Authors: Kraskouski, A. N., Nikalaichuk, V. V., Hileuskaya, K. S., Kulikouskaya, V. I., Stepanova, E. A., Kuzminski, I. I., Opavski, N., Agabekov, V. E.
• Format: Article
• Language: English
• Subjects: Antibiotic resistance; Antibiotics; Antimicrobial agents; Binding; Biochemistry; Biomedical and Life Sciences; Cefotaxime; Ceftriaxone; Cephalosporins; Chemical reduction; Chemical synthesis; Chitosan; E coli; Green chemistry; Infrared spectroscopy; Life Sciences; Medical Microbiology; Microbiology; Mixtures; Nanocomposites; Nanoparticles; Silver; Silver nitrate
• ISSN: 0003-6838; 1608-3024
• DOI: 10.1134/S0003683822020120

Aggregatively stable, positively charged (~45 mV) chitosan-Ag nanocomposites with a spherical shape and size up to 60.0 nm were synthesized by the “green chemistry” method via the chemical reduction of silver nitrate with chitosan. Chitosan-Ag/ceftriaxone and chitosan-Ag/cefotaxime complexes containing up to 1.06 ± 0.01 and 1.29 ± 0.03 mg antibiotic/mg chitosan-Ag respectively were obtained. It was found that an increase in the ceftriaxone : chitosan-Ag weight ratio in the reaction mixture from 0.5 to 1.25 leads to an increase in the efficiency of antibiotic binding to nanoparticles from 7.2 ± 0.2 to 71.7 ± 0.2%. With an increase in the cefotaxime : chitosan-Ag ratio in the reaction mixture from 0.5 to 10, the antibiotic binding efficiency decreases from 66.2 ± 1.5 to 13.0 ± 0.7%. The formation of chitosan-Ag/antibiotic complexes was confirmed by infrared (IR) spectroscopy. The effectiveness of the use of the chitosan-Ag/cefotaxime complexes against antibiotic-resistant strains of Escherichia coli and Staphylococcus aureus was established.