Microbiological Analysis of the Cloth Bundle Exposed on the External Surface of the International Space Station Over 8 Years

As a part of the space experiment TEST, the cloth bundle was placed on the external surface of the International Space Station (ISS) and returned to Earth after 8 years of exposure to the open space. Microbiological investigation of the cloth resulted in the detection of viable yeasts. Analysis of n...

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Bibliographic Details
Published in:BioNanoScience 2022-03, Vol.12 (1), p.142-148
Main Authors: Deshevaya, Elena A., Kachalkin, Aleksey V., Maksimova, Irina A., Fialkina, Svetlana V., Shubralova, Elena V., Tsygankov, Oleg S., Ilyin, Vyacheslav К., Orlov, Oleg I., Morzunov, Sergey P., Nikolaeva, Irina V.
Format: Article
Language:English
Subjects:
Biological and Medical Physics
Biomaterials
Biophysics
Circuits and Systems
Cloth
Clotrimazole
DNA fingerprinting
Engineering
Exposure
Fungicides
International Space Station
Ketoconazole
Molecular
Multinational space ventures
Nanotechnology
Nucleotides
Precision and Regenerative Medicine - 2021
Space stations
Yeast
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Summary:As a part of the space experiment TEST, the cloth bundle was placed on the external surface of the International Space Station (ISS) and returned to Earth after 8 years of exposure to the open space. Microbiological investigation of the cloth resulted in the detection of viable yeasts. Analysis of nucleotide sequences of the rDNA ITS region established that the yeasts belonged to Rhodotorula mucilaginosa . Microsatellite DNA profiling evidenced likeness of the strain isolated from the ISS interior in 2007 and the strain that contaminated the cloth bundle. Physiological testing also revealed significant similarities and some differences of the strain found on the cloth and the cultured yeast strain previously detected in the ISS interior. This investigation demonstrated that the fungal strains recovered from either the ISS interior or from the cloth exposed to outer space had changed sensitivity to antifungal drugs clotrimazole and ketoconazole depending on the number of passages.
ISSN:2191-1630
2191-1649
DOI:10.1007/s12668-021-00930-6