First report of a ‘Candidatus Phytoplasma asteris’ strain affecting lingonberry (Vaccinium vitis‐idaea) and causing lingonberry stunt phytoplasma disease

Lingonberry plants analysed in this study: (a) asymptomatic lingonberry plant with normal growth and development; (b) lingonberry plant showing typical symptoms of lingonberry stunt phytoplasma disease including stunted growth, little leaves, and yellowing TABLE 1 Lingonberry samples collected in Qu...

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Published in:New disease reports 2022-01, Vol.45 (1), p.n/a
Main Authors: Brochu, A.‐S., Methot, A., Breton, A.‐M., Lacroix, C., Légaré, J.‐P., Pérez‐López, E.
Format: Article
Language:English
Subjects:
16SrI
aster yellows phytoplasma
Asymptomatic
Cloning
Cultivars
Deoxyribonucleic acid
DNA
LbSP
Leaves
Phylogenetics
Phylogeny
Phytoplasma
Plant bacterial diseases
Polymerase chain reaction
rRNA 16S
Stunt
Vaccinium vitis-idaea
Yellowing
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Pérez‐López, E.
description Lingonberry plants analysed in this study: (a) asymptomatic lingonberry plant with normal growth and development; (b) lingonberry plant showing typical symptoms of lingonberry stunt phytoplasma disease including stunted growth, little leaves, and yellowing TABLE 1 Lingonberry samples collected in Quebec. Canada analysed in this study Cultivar Symptoms Sample Tissue Date of sample preparation Date of DNA extraction [DNA] ng/μL A260/A280 A260/A230 Cpn60 PCR 16S PCR Sussi Little leaves and yellowing 1 Leaf 9-9-21 12-10-21 409.1 1.95 1.04 Neg Neg 2 Stem 9-9-21 15-11-21 892.4 2.10 1.78 Pos Pos Magenta Little leaves and yellowing 3 Leaf 9-9-21 12-10-21 189.1 1.85 0.89 Neg Neg 4 Stem 9-9-21 15-11-21 981.2 2.09 1.78 Pos Pos Koralle Little leaves and yellowing 5 Leaf 9-9-21 12-10-21 433.7 1.98 1.11 Neg Neg 6 Stem 9-9-21 15-11-21 732.7 2.11 1.75 Pos Pos Sussi Asymptomatic 7 Leaf 9-9-21 12-10-21 186.5 1.89 0.99 Neg Neg 8 Stem 9-9-21 15-11-21 560 2.07 1.45 Pos Pos Magenta Asymptomatic 9 Leaf 9-9-21 12-10-21 142.7 1.75 0.74 Neg Neg 10 Stem 9-9-21 15-11-21 211.5 1.97 1.16 Neg Neg Koralle Asymptomatic 11 Leaf 9-9-21 12-10-21 170.2 1.91 0.93 Neg Neg 12 Stem 9-9-21 15-11-21 673.6 2.06 1.65 Pos Pos Koralle Little leaves and stunted plants 13 Leaf 9-9-21 20-10-21 546.9 1.98 1.21 Neg Neg 14 Leaf 9-9-21 14-9-21 1512.5 2.01 1.8 Pos Pos 15 Stem 9-9-21 15-11-21 2909.5 2.12 1.72 Neg Neg Magenta Little leaves and yellowing 16 Leaf 9-9-21 15-11-21 228.3 1.51 0.69 Pos Pos 17 Leaf 9-9-21 14-9-21 184 1.47 0.63 Neg Neg 18 Stem 9-9-21 15-11-21 3824.8 1.98 0.94 Neg Neg Magenta Little leaves and yellowing 19 Leaf 9-9-21 12-10-21 324.4 0.91 0.27 Neg Neg 20 Stem 9-9-21 15-11-21 1047.8 1.94 0.94 Neg Neg Koralle Little leaves and stunted plants 21 Leaf 9-9-21 12-10-21 142.4 1.13 0.30 Neg Neg 22 Stem 9-9-21 15-11-21 3418.8 1.94 0.96 Neg Neg Koralle Little leaves and stunted plants 23 Leaf 9-9-21 12-10-21 195.7 1.17 0.39 Neg Neg 24 Stem 9-9-21 15-11-21 631.2 1.92 1.06 Neg Neg Koralle Little leaves and stunted plants 25 Leaf 9-9-21 20-10-21 307.6 0.92 0.25 Neg Neg 26 Leaf 9-9-21 14-9-21 364 1.73 0.94 Neg Neg 27 Stem 9-9-21 15-11-21 1670.7 1.83 0.54 Neg Neg Erntesegen Asymptomatic 28 Leaf 9-9-21 20-10-21 101.7 1.13 0.36 Neg Neg 29 Leaf 9-9-21 15-11-21 389 1.68 0.94 Neg Neg 30 Stem 9-9-21 15-11-21 1366.8 2.03 1.3 Pos Neg Koralle Asymptomatic 31 Leaf 9-9-21 20-10-21 139.4 0.88 0.27 Neg Neg 32 Leaf 9-9-21 15-11-21 196.1 1.55 0.89 Neg Neg 33 Stem 9-9-21 15-11-21 1697 2.00 1.19 Neg Neg Koralle Asymptom
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Canada analysed in this study Cultivar Symptoms Sample Tissue Date of sample preparation Date of DNA extraction [DNA] ng/μL A260/A280 A260/A230 Cpn60 PCR 16S PCR Sussi Little leaves and yellowing 1 Leaf 9-9-21 12-10-21 409.1 1.95 1.04 Neg Neg 2 Stem 9-9-21 15-11-21 892.4 2.10 1.78 Pos Pos Magenta Little leaves and yellowing 3 Leaf 9-9-21 12-10-21 189.1 1.85 0.89 Neg Neg 4 Stem 9-9-21 15-11-21 981.2 2.09 1.78 Pos Pos Koralle Little leaves and yellowing 5 Leaf 9-9-21 12-10-21 433.7 1.98 1.11 Neg Neg 6 Stem 9-9-21 15-11-21 732.7 2.11 1.75 Pos Pos Sussi Asymptomatic 7 Leaf 9-9-21 12-10-21 186.5 1.89 0.99 Neg Neg 8 Stem 9-9-21 15-11-21 560 2.07 1.45 Pos Pos Magenta Asymptomatic 9 Leaf 9-9-21 12-10-21 142.7 1.75 0.74 Neg Neg 10 Stem 9-9-21 15-11-21 211.5 1.97 1.16 Neg Neg Koralle Asymptomatic 11 Leaf 9-9-21 12-10-21 170.2 1.91 0.93 Neg Neg 12 Stem 9-9-21 15-11-21 673.6 2.06 1.65 Pos Pos Koralle Little leaves and stunted plants 13 Leaf 9-9-21 20-10-21 546.9 1.98 1.21 Neg Neg 14 Leaf 9-9-21 14-9-21 1512.5 2.01 1.8 Pos Pos 15 Stem 9-9-21 15-11-21 2909.5 2.12 1.72 Neg Neg Magenta Little leaves and yellowing 16 Leaf 9-9-21 15-11-21 228.3 1.51 0.69 Pos Pos 17 Leaf 9-9-21 14-9-21 184 1.47 0.63 Neg Neg 18 Stem 9-9-21 15-11-21 3824.8 1.98 0.94 Neg Neg Magenta Little leaves and yellowing 19 Leaf 9-9-21 12-10-21 324.4 0.91 0.27 Neg Neg 20 Stem 9-9-21 15-11-21 1047.8 1.94 0.94 Neg Neg Koralle Little leaves and stunted plants 21 Leaf 9-9-21 12-10-21 142.4 1.13 0.30 Neg Neg 22 Stem 9-9-21 15-11-21 3418.8 1.94 0.96 Neg Neg Koralle Little leaves and stunted plants 23 Leaf 9-9-21 12-10-21 195.7 1.17 0.39 Neg Neg 24 Stem 9-9-21 15-11-21 631.2 1.92 1.06 Neg Neg Koralle Little leaves and stunted plants 25 Leaf 9-9-21 20-10-21 307.6 0.92 0.25 Neg Neg 26 Leaf 9-9-21 14-9-21 364 1.73 0.94 Neg Neg 27 Stem 9-9-21 15-11-21 1670.7 1.83 0.54 Neg Neg Erntesegen Asymptomatic 28 Leaf 9-9-21 20-10-21 101.7 1.13 0.36 Neg Neg 29 Leaf 9-9-21 15-11-21 389 1.68 0.94 Neg Neg 30 Stem 9-9-21 15-11-21 1366.8 2.03 1.3 Pos Neg Koralle Asymptomatic 31 Leaf 9-9-21 20-10-21 139.4 0.88 0.27 Neg Neg 32 Leaf 9-9-21 15-11-21 196.1 1.55 0.89 Neg Neg 33 Stem 9-9-21 15-11-21 1697 2.00 1.19 Neg Neg Koralle Asymptomatic 34 Leaf 9-9-21 12-10-21 226.8 1.76 0.87 Neg Neg 35 Stem 9-9-21 15-11-21 704.7 2.08 1.46 Pos Pos FIGURE 2. Phylogenetic tree based on the 16S rRNA gene sequences of the lingonberry stunt phytoplasma (LbSP) strains identified in this study and ‘Ca.</description><identifier>ISSN: 2044-0588</identifier><identifier>EISSN: 2044-0588</identifier><identifier>DOI: 10.1002/ndr2.12058</identifier><language>eng</language><publisher>Reading: John Wiley &amp; Sons, Inc</publisher><subject>16SrI ; aster yellows phytoplasma ; Asymptomatic ; Cloning ; Cultivars ; Deoxyribonucleic acid ; DNA ; LbSP ; Leaves ; Phylogenetics ; Phylogeny ; Phytoplasma ; Plant bacterial diseases ; Polymerase chain reaction ; rRNA 16S ; Stunt ; Vaccinium vitis-idaea ; Yellowing</subject><ispartof>New disease reports, 2022-01, Vol.45 (1), p.n/a</ispartof><rights>2022 The Authors. published by British Society for Plant Pathology and John Wiley &amp; Sons</rights><rights>2022. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). 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Canada analysed in this study Cultivar Symptoms Sample Tissue Date of sample preparation Date of DNA extraction [DNA] ng/μL A260/A280 A260/A230 Cpn60 PCR 16S PCR Sussi Little leaves and yellowing 1 Leaf 9-9-21 12-10-21 409.1 1.95 1.04 Neg Neg 2 Stem 9-9-21 15-11-21 892.4 2.10 1.78 Pos Pos Magenta Little leaves and yellowing 3 Leaf 9-9-21 12-10-21 189.1 1.85 0.89 Neg Neg 4 Stem 9-9-21 15-11-21 981.2 2.09 1.78 Pos Pos Koralle Little leaves and yellowing 5 Leaf 9-9-21 12-10-21 433.7 1.98 1.11 Neg Neg 6 Stem 9-9-21 15-11-21 732.7 2.11 1.75 Pos Pos Sussi Asymptomatic 7 Leaf 9-9-21 12-10-21 186.5 1.89 0.99 Neg Neg 8 Stem 9-9-21 15-11-21 560 2.07 1.45 Pos Pos Magenta Asymptomatic 9 Leaf 9-9-21 12-10-21 142.7 1.75 0.74 Neg Neg 10 Stem 9-9-21 15-11-21 211.5 1.97 1.16 Neg Neg Koralle Asymptomatic 11 Leaf 9-9-21 12-10-21 170.2 1.91 0.93 Neg Neg 12 Stem 9-9-21 15-11-21 673.6 2.06 1.65 Pos Pos Koralle Little leaves and stunted plants 13 Leaf 9-9-21 20-10-21 546.9 1.98 1.21 Neg Neg 14 Leaf 9-9-21 14-9-21 1512.5 2.01 1.8 Pos Pos 15 Stem 9-9-21 15-11-21 2909.5 2.12 1.72 Neg Neg Magenta Little leaves and yellowing 16 Leaf 9-9-21 15-11-21 228.3 1.51 0.69 Pos Pos 17 Leaf 9-9-21 14-9-21 184 1.47 0.63 Neg Neg 18 Stem 9-9-21 15-11-21 3824.8 1.98 0.94 Neg Neg Magenta Little leaves and yellowing 19 Leaf 9-9-21 12-10-21 324.4 0.91 0.27 Neg Neg 20 Stem 9-9-21 15-11-21 1047.8 1.94 0.94 Neg Neg Koralle Little leaves and stunted plants 21 Leaf 9-9-21 12-10-21 142.4 1.13 0.30 Neg Neg 22 Stem 9-9-21 15-11-21 3418.8 1.94 0.96 Neg Neg Koralle Little leaves and stunted plants 23 Leaf 9-9-21 12-10-21 195.7 1.17 0.39 Neg Neg 24 Stem 9-9-21 15-11-21 631.2 1.92 1.06 Neg Neg Koralle Little leaves and stunted plants 25 Leaf 9-9-21 20-10-21 307.6 0.92 0.25 Neg Neg 26 Leaf 9-9-21 14-9-21 364 1.73 0.94 Neg Neg 27 Stem 9-9-21 15-11-21 1670.7 1.83 0.54 Neg Neg Erntesegen Asymptomatic 28 Leaf 9-9-21 20-10-21 101.7 1.13 0.36 Neg Neg 29 Leaf 9-9-21 15-11-21 389 1.68 0.94 Neg Neg 30 Stem 9-9-21 15-11-21 1366.8 2.03 1.3 Pos Neg Koralle Asymptomatic 31 Leaf 9-9-21 20-10-21 139.4 0.88 0.27 Neg Neg 32 Leaf 9-9-21 15-11-21 196.1 1.55 0.89 Neg Neg 33 Stem 9-9-21 15-11-21 1697 2.00 1.19 Neg Neg Koralle Asymptomatic 34 Leaf 9-9-21 12-10-21 226.8 1.76 0.87 Neg Neg 35 Stem 9-9-21 15-11-21 704.7 2.08 1.46 Pos Pos FIGURE 2. Phylogenetic tree based on the 16S rRNA gene sequences of the lingonberry stunt phytoplasma (LbSP) strains identified in this study and ‘Ca.</description><subject>16SrI</subject><subject>aster yellows phytoplasma</subject><subject>Asymptomatic</subject><subject>Cloning</subject><subject>Cultivars</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>LbSP</subject><subject>Leaves</subject><subject>Phylogenetics</subject><subject>Phylogeny</subject><subject>Phytoplasma</subject><subject>Plant bacterial diseases</subject><subject>Polymerase chain reaction</subject><subject>rRNA 16S</subject><subject>Stunt</subject><subject>Vaccinium vitis-idaea</subject><subject>Yellowing</subject><issn>2044-0588</issn><issn>2044-0588</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>24P</sourceid><sourceid>PIMPY</sourceid><recordid>eNp9kctKw0AUhoMoWKsbn2DAjQqpc8l1KdWqUFRE3YaTmYlOSZM4M1Gy6yO41JXv1idxalwUF27OBb7_P3B-z9sneEQwpieV0HREKA6TDW9AcRD4bkw21-Ztb8eYGXYrScnA-5oobSzSsqm1RXWBAC0XH2OohBJgW4NunztbNyWYOSAwVmpllotPZKwGVSEoCsmtqp5Q6Upd5VLrDh0-AueqUu0cvSq7Erw7NwlHyPkiDq35qzC2rSxq1m4JZSQYuettFVAauffbh97D5Px-fOlPby6uxqdTnzOSJr7kLA9oHgecFcJ9IqfAQNKQF2EcxiDCKMaEyYjwPCcsIVSIJMZplFIaJSkGNvQOet9G1y-tNDab1a2u3MmMRkHASIhp5KjjnuK6NkbLImu0moPuMoKzVQDZKoDsJwAHkx5-U6Xs_iGz67M72mu-AUbQjfM</recordid><startdate>202201</startdate><enddate>202201</enddate><creator>Brochu, A.‐S.</creator><creator>Methot, A.</creator><creator>Breton, A.‐M.</creator><creator>Lacroix, C.</creator><creator>Légaré, J.‐P.</creator><creator>Pérez‐López, E.</creator><general>John Wiley &amp; 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(b) lingonberry plant showing typical symptoms of lingonberry stunt phytoplasma disease including stunted growth, little leaves, and yellowing TABLE 1 Lingonberry samples collected in Quebec. Canada analysed in this study Cultivar Symptoms Sample Tissue Date of sample preparation Date of DNA extraction [DNA] ng/μL A260/A280 A260/A230 Cpn60 PCR 16S PCR Sussi Little leaves and yellowing 1 Leaf 9-9-21 12-10-21 409.1 1.95 1.04 Neg Neg 2 Stem 9-9-21 15-11-21 892.4 2.10 1.78 Pos Pos Magenta Little leaves and yellowing 3 Leaf 9-9-21 12-10-21 189.1 1.85 0.89 Neg Neg 4 Stem 9-9-21 15-11-21 981.2 2.09 1.78 Pos Pos Koralle Little leaves and yellowing 5 Leaf 9-9-21 12-10-21 433.7 1.98 1.11 Neg Neg 6 Stem 9-9-21 15-11-21 732.7 2.11 1.75 Pos Pos Sussi Asymptomatic 7 Leaf 9-9-21 12-10-21 186.5 1.89 0.99 Neg Neg 8 Stem 9-9-21 15-11-21 560 2.07 1.45 Pos Pos Magenta Asymptomatic 9 Leaf 9-9-21 12-10-21 142.7 1.75 0.74 Neg Neg 10 Stem 9-9-21 15-11-21 211.5 1.97 1.16 Neg Neg Koralle Asymptomatic 11 Leaf 9-9-21 12-10-21 170.2 1.91 0.93 Neg Neg 12 Stem 9-9-21 15-11-21 673.6 2.06 1.65 Pos Pos Koralle Little leaves and stunted plants 13 Leaf 9-9-21 20-10-21 546.9 1.98 1.21 Neg Neg 14 Leaf 9-9-21 14-9-21 1512.5 2.01 1.8 Pos Pos 15 Stem 9-9-21 15-11-21 2909.5 2.12 1.72 Neg Neg Magenta Little leaves and yellowing 16 Leaf 9-9-21 15-11-21 228.3 1.51 0.69 Pos Pos 17 Leaf 9-9-21 14-9-21 184 1.47 0.63 Neg Neg 18 Stem 9-9-21 15-11-21 3824.8 1.98 0.94 Neg Neg Magenta Little leaves and yellowing 19 Leaf 9-9-21 12-10-21 324.4 0.91 0.27 Neg Neg 20 Stem 9-9-21 15-11-21 1047.8 1.94 0.94 Neg Neg Koralle Little leaves and stunted plants 21 Leaf 9-9-21 12-10-21 142.4 1.13 0.30 Neg Neg 22 Stem 9-9-21 15-11-21 3418.8 1.94 0.96 Neg Neg Koralle Little leaves and stunted plants 23 Leaf 9-9-21 12-10-21 195.7 1.17 0.39 Neg Neg 24 Stem 9-9-21 15-11-21 631.2 1.92 1.06 Neg Neg Koralle Little leaves and stunted plants 25 Leaf 9-9-21 20-10-21 307.6 0.92 0.25 Neg Neg 26 Leaf 9-9-21 14-9-21 364 1.73 0.94 Neg Neg 27 Stem 9-9-21 15-11-21 1670.7 1.83 0.54 Neg Neg Erntesegen Asymptomatic 28 Leaf 9-9-21 20-10-21 101.7 1.13 0.36 Neg Neg 29 Leaf 9-9-21 15-11-21 389 1.68 0.94 Neg Neg 30 Stem 9-9-21 15-11-21 1366.8 2.03 1.3 Pos Neg Koralle Asymptomatic 31 Leaf 9-9-21 20-10-21 139.4 0.88 0.27 Neg Neg 32 Leaf 9-9-21 15-11-21 196.1 1.55 0.89 Neg Neg 33 Stem 9-9-21 15-11-21 1697 2.00 1.19 Neg Neg Koralle Asymptomatic 34 Leaf 9-9-21 12-10-21 226.8 1.76 0.87 Neg Neg 35 Stem 9-9-21 15-11-21 704.7 2.08 1.46 Pos Pos FIGURE 2. Phylogenetic tree based on the 16S rRNA gene sequences of the lingonberry stunt phytoplasma (LbSP) strains identified in this study and ‘Ca.</abstract><cop>Reading</cop><pub>John Wiley &amp; Sons, Inc</pub><doi>10.1002/ndr2.12058</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record>
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identifier ISSN: 2044-0588
ispartof New disease reports, 2022-01, Vol.45 (1), p.n/a
issn 2044-0588
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language eng
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source Publicly Available Content Database (Proquest) (PQ_SDU_P3); Wiley Online Library Open Access
subjects 16SrI
aster yellows phytoplasma
Asymptomatic
Cloning
Cultivars
Deoxyribonucleic acid
DNA
LbSP
Leaves
Phylogenetics
Phylogeny
Phytoplasma
Plant bacterial diseases
Polymerase chain reaction
rRNA 16S
Stunt
Vaccinium vitis-idaea
Yellowing
title First report of a ‘Candidatus Phytoplasma asteris’ strain affecting lingonberry (Vaccinium vitis‐idaea) and causing lingonberry stunt phytoplasma disease
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