Real-time PCR-based identification methods for Megaselia scalaris (Loew) (Diptera: Phoridae) targeting mitochondrial DNA

The scuttle fly, Megaselia scalaris (Loew, 1866) (Diptera: Phoridae), is an insect of the family Dermatidae that often poses problems as a foreign substance contaminating foods. In this study, we developed a rapid identification method for this contaminant using mitochondrial DNA based on a molecula...

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Bibliographic Details
Published in:FOOD SCIENCE AND TECHNOLOGY RESEARCH 2022, Vol.28(2), pp.119-122
Main Authors: Furui, Satoshi, Miyanoshita, Akihiro, Imamura, Taro
Format: Article
Language:English
Subjects:
Contaminants
Diptera
Food contamination
identification
Identification methods
Insects
Megaselia scalaris
Mitochondrial DNA
Molecular biology
Pests
Phoridae
Polymerase chain reaction
Real time
small fly
trap
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Summary:The scuttle fly, Megaselia scalaris (Loew, 1866) (Diptera: Phoridae), is an insect of the family Dermatidae that often poses problems as a foreign substance contaminating foods. In this study, we developed a rapid identification method for this contaminant using mitochondrial DNA based on a molecular biology technique. From the entire region of mitochondrial DNA information, a region suitable for real-time polymerase chain reaction (PCR) was chosen, and two primers and a probe specific to M. scalaris were designed. A specificity test was performed with 16 species of pests that could be contaminants in food, and the developed real-time PCR method was confirmed to show a positive result only for M. scalaris. In addition, the limit of detection of this analysis method using a 10-µL PCR solution was 1 pg of total DNA, which revealed that the method was highly sensitive.
ISSN:1344-6606
1881-3984
DOI:10.3136/fstr.FSTR-D-21-00241