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Comparison of cytochrome P450 CYP332A1 gene in resistant and susceptible strains of the cotton bollworm, Helicoverpa armigera (Lepidoptera: Noctuidae)
The cotton bollworm, Helicoverpa armigera Hübner (Lepidoptera: Noctuidae), an important polyphagous pest, has shown different levels of resistance to various insecticides. Cytochrome P450 monooxygenases (P450s) are known to be closely related to insecticide resistance of H. armigera , but its intern...
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| Published in: | Applied entomology and zoology 2022-05, Vol.57 (2), p.171-181 |
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| Main Authors: | , , , , |
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| cites | cdi_FETCH-LOGICAL-c346t-5ff9dd66e9643af50d3704253c430ebd272622902ec0e1bd5e9a03c7390ff9353 |
| container_end_page | 181 |
| container_issue | 2 |
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| container_title | Applied entomology and zoology |
| container_volume | 57 |
| creator | Huang, Yun Qin, Jianying Wu, Peizhuo Zheng, Junyue Qiu, Lihong |
| description | The cotton bollworm,
Helicoverpa armigera
Hübner (Lepidoptera: Noctuidae), an important polyphagous pest, has shown different levels of resistance to various insecticides. Cytochrome P450 monooxygenases (P450s) are known to be closely related to insecticide resistance of
H. armigera
, but its internal mechanism is still not completely understood. In this study,
CYP332A1
was cloned and characterized from fenvalerate-resistant (BJ) and susceptible (HDS) strains of
H. armigera
. The cDNA sequence of
CYP332A1
contains an open reading frame (ORF) of 1509 bp encoding 502 amino acid residues. The expression level of
CYP332A1
in BJ strain was significantly higher than that in HDS strain, and five amino acid mutations (F21C, G28R, K64Q, A290S and V477I) were observed in the sequence of BJ strain. The 5ʹ-flanking sequence of
CYP332A1
contains the binding sites of putative transcription factors of Dr, Ubx, Cf2, caup, ara, Antp, ftz, eve and otp, and there were three large deletions of 386–392 bp, 547–554 bp and 965–971 bp in the 5ʹ-flanking sequence of
CYP332A1
of BJ strain. The results suggested that
CYP332A1
might play an important role in the resistance of
H. armigera
to fenvalerate, and the deletion of 5ʹ-flanking sequence of
CYP332A1
might be one of the reasons for the development of resistance of
H. armigera
to fenvalerate. |
| doi_str_mv | 10.1007/s13355-022-00777-7 |
| format | article |
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Helicoverpa armigera
Hübner (Lepidoptera: Noctuidae), an important polyphagous pest, has shown different levels of resistance to various insecticides. Cytochrome P450 monooxygenases (P450s) are known to be closely related to insecticide resistance of
H. armigera
, but its internal mechanism is still not completely understood. In this study,
CYP332A1
was cloned and characterized from fenvalerate-resistant (BJ) and susceptible (HDS) strains of
H. armigera
. The cDNA sequence of
CYP332A1
contains an open reading frame (ORF) of 1509 bp encoding 502 amino acid residues. The expression level of
CYP332A1
in BJ strain was significantly higher than that in HDS strain, and five amino acid mutations (F21C, G28R, K64Q, A290S and V477I) were observed in the sequence of BJ strain. The 5ʹ-flanking sequence of
CYP332A1
contains the binding sites of putative transcription factors of Dr, Ubx, Cf2, caup, ara, Antp, ftz, eve and otp, and there were three large deletions of 386–392 bp, 547–554 bp and 965–971 bp in the 5ʹ-flanking sequence of
CYP332A1
of BJ strain. The results suggested that
CYP332A1
might play an important role in the resistance of
H. armigera
to fenvalerate, and the deletion of 5ʹ-flanking sequence of
CYP332A1
might be one of the reasons for the development of resistance of
H. armigera
to fenvalerate.</description><identifier>ISSN: 0003-6862</identifier><identifier>EISSN: 1347-605X</identifier><identifier>DOI: 10.1007/s13355-022-00777-7</identifier><language>eng</language><publisher>Tokyo: Springer Japan</publisher><subject>Agriculture ; Applied Ecology ; Binding sites ; Biomedical and Life Sciences ; Cytochrome ; Cytochrome P450 ; Entomology ; Environmental Management ; Fenvalerate ; Helicoverpa armigera ; Insecticides ; Lepidoptera ; Life Sciences ; Noctuidae ; Open reading frames ; Original Research Paper ; Pesticide resistance ; Plant Pathology ; Transcription factors ; Zoology</subject><ispartof>Applied entomology and zoology, 2022-05, Vol.57 (2), p.171-181</ispartof><rights>The Author(s) under exclusive licence to The Japanese Society of Applied Entomology and Zoology 2022</rights><rights>The Author(s) under exclusive licence to The Japanese Society of Applied Entomology and Zoology 2022.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c346t-5ff9dd66e9643af50d3704253c430ebd272622902ec0e1bd5e9a03c7390ff9353</citedby><cites>FETCH-LOGICAL-c346t-5ff9dd66e9643af50d3704253c430ebd272622902ec0e1bd5e9a03c7390ff9353</cites><orcidid>0000-0002-4635-7641</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27923,27924</link.rule.ids></links><search><creatorcontrib>Huang, Yun</creatorcontrib><creatorcontrib>Qin, Jianying</creatorcontrib><creatorcontrib>Wu, Peizhuo</creatorcontrib><creatorcontrib>Zheng, Junyue</creatorcontrib><creatorcontrib>Qiu, Lihong</creatorcontrib><title>Comparison of cytochrome P450 CYP332A1 gene in resistant and susceptible strains of the cotton bollworm, Helicoverpa armigera (Lepidoptera: Noctuidae)</title><title>Applied entomology and zoology</title><addtitle>Appl Entomol Zool</addtitle><description>The cotton bollworm,
Helicoverpa armigera
Hübner (Lepidoptera: Noctuidae), an important polyphagous pest, has shown different levels of resistance to various insecticides. Cytochrome P450 monooxygenases (P450s) are known to be closely related to insecticide resistance of
H. armigera
, but its internal mechanism is still not completely understood. In this study,
CYP332A1
was cloned and characterized from fenvalerate-resistant (BJ) and susceptible (HDS) strains of
H. armigera
. The cDNA sequence of
CYP332A1
contains an open reading frame (ORF) of 1509 bp encoding 502 amino acid residues. The expression level of
CYP332A1
in BJ strain was significantly higher than that in HDS strain, and five amino acid mutations (F21C, G28R, K64Q, A290S and V477I) were observed in the sequence of BJ strain. The 5ʹ-flanking sequence of
CYP332A1
contains the binding sites of putative transcription factors of Dr, Ubx, Cf2, caup, ara, Antp, ftz, eve and otp, and there were three large deletions of 386–392 bp, 547–554 bp and 965–971 bp in the 5ʹ-flanking sequence of
CYP332A1
of BJ strain. The results suggested that
CYP332A1
might play an important role in the resistance of
H. armigera
to fenvalerate, and the deletion of 5ʹ-flanking sequence of
CYP332A1
might be one of the reasons for the development of resistance of
H. armigera
to fenvalerate.</description><subject>Agriculture</subject><subject>Applied Ecology</subject><subject>Binding sites</subject><subject>Biomedical and Life Sciences</subject><subject>Cytochrome</subject><subject>Cytochrome P450</subject><subject>Entomology</subject><subject>Environmental Management</subject><subject>Fenvalerate</subject><subject>Helicoverpa armigera</subject><subject>Insecticides</subject><subject>Lepidoptera</subject><subject>Life Sciences</subject><subject>Noctuidae</subject><subject>Open reading frames</subject><subject>Original Research Paper</subject><subject>Pesticide resistance</subject><subject>Plant Pathology</subject><subject>Transcription factors</subject><subject>Zoology</subject><issn>0003-6862</issn><issn>1347-605X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><recordid>eNp9kE1LHEEQhpsQIevqH_DUkItCRmv6c8ebLDEKS_SgoKemt6dGW2amJ929in_E35vebCC3nIoX6nmLegg5quG0BtBnqeZcygoYq0rUutKfyKzmQlcK5MNnMgMAXqmFYl_IfkovAEw0CzEjH8swTDb6FEYaOurec3DPMQxIb4UEuny85Zxd1PQJR6R-pBGTT9mOmdqxpWmTHE7Zr3ukKUfrx7Rtyc9IXci5dK5D37-FOHyjV9h7F14xTpbaOPgnjJYer3DybZhyCef0Z3B541uLJwdkr7N9wsO_c07uL7_fLa-q1c2P6-XFqnJcqFzJrmvaVilslOC2k9ByDYJJ7gQHXLdMM8VYAwwdYL1uJTYWuNO8gUJyyefk6653iuHXBlM2L2ETx3LSMCW0WsBW3Jyw3ZaLIaWInZmiH2x8NzWYrX-z82-Kf_PHv9EF4jsoleWxfPuv-j_Ub0oqiRU</recordid><startdate>20220501</startdate><enddate>20220501</enddate><creator>Huang, Yun</creator><creator>Qin, Jianying</creator><creator>Wu, Peizhuo</creator><creator>Zheng, Junyue</creator><creator>Qiu, Lihong</creator><general>Springer Japan</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7SS</scope><orcidid>https://orcid.org/0000-0002-4635-7641</orcidid></search><sort><creationdate>20220501</creationdate><title>Comparison of cytochrome P450 CYP332A1 gene in resistant and susceptible strains of the cotton bollworm, Helicoverpa armigera (Lepidoptera: Noctuidae)</title><author>Huang, Yun ; Qin, Jianying ; Wu, Peizhuo ; Zheng, Junyue ; Qiu, Lihong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c346t-5ff9dd66e9643af50d3704253c430ebd272622902ec0e1bd5e9a03c7390ff9353</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Agriculture</topic><topic>Applied Ecology</topic><topic>Binding sites</topic><topic>Biomedical and Life Sciences</topic><topic>Cytochrome</topic><topic>Cytochrome P450</topic><topic>Entomology</topic><topic>Environmental Management</topic><topic>Fenvalerate</topic><topic>Helicoverpa armigera</topic><topic>Insecticides</topic><topic>Lepidoptera</topic><topic>Life Sciences</topic><topic>Noctuidae</topic><topic>Open reading frames</topic><topic>Original Research Paper</topic><topic>Pesticide resistance</topic><topic>Plant Pathology</topic><topic>Transcription factors</topic><topic>Zoology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Huang, Yun</creatorcontrib><creatorcontrib>Qin, Jianying</creatorcontrib><creatorcontrib>Wu, Peizhuo</creatorcontrib><creatorcontrib>Zheng, Junyue</creatorcontrib><creatorcontrib>Qiu, Lihong</creatorcontrib><collection>CrossRef</collection><collection>Entomology Abstracts (Full archive)</collection><jtitle>Applied entomology and zoology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Huang, Yun</au><au>Qin, Jianying</au><au>Wu, Peizhuo</au><au>Zheng, Junyue</au><au>Qiu, Lihong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparison of cytochrome P450 CYP332A1 gene in resistant and susceptible strains of the cotton bollworm, Helicoverpa armigera (Lepidoptera: Noctuidae)</atitle><jtitle>Applied entomology and zoology</jtitle><stitle>Appl Entomol Zool</stitle><date>2022-05-01</date><risdate>2022</risdate><volume>57</volume><issue>2</issue><spage>171</spage><epage>181</epage><pages>171-181</pages><issn>0003-6862</issn><eissn>1347-605X</eissn><abstract>The cotton bollworm,
Helicoverpa armigera
Hübner (Lepidoptera: Noctuidae), an important polyphagous pest, has shown different levels of resistance to various insecticides. Cytochrome P450 monooxygenases (P450s) are known to be closely related to insecticide resistance of
H. armigera
, but its internal mechanism is still not completely understood. In this study,
CYP332A1
was cloned and characterized from fenvalerate-resistant (BJ) and susceptible (HDS) strains of
H. armigera
. The cDNA sequence of
CYP332A1
contains an open reading frame (ORF) of 1509 bp encoding 502 amino acid residues. The expression level of
CYP332A1
in BJ strain was significantly higher than that in HDS strain, and five amino acid mutations (F21C, G28R, K64Q, A290S and V477I) were observed in the sequence of BJ strain. The 5ʹ-flanking sequence of
CYP332A1
contains the binding sites of putative transcription factors of Dr, Ubx, Cf2, caup, ara, Antp, ftz, eve and otp, and there were three large deletions of 386–392 bp, 547–554 bp and 965–971 bp in the 5ʹ-flanking sequence of
CYP332A1
of BJ strain. The results suggested that
CYP332A1
might play an important role in the resistance of
H. armigera
to fenvalerate, and the deletion of 5ʹ-flanking sequence of
CYP332A1
might be one of the reasons for the development of resistance of
H. armigera
to fenvalerate.</abstract><cop>Tokyo</cop><pub>Springer Japan</pub><doi>10.1007/s13355-022-00777-7</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0002-4635-7641</orcidid></addata></record> |
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| ispartof | Applied entomology and zoology, 2022-05, Vol.57 (2), p.171-181 |
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| language | eng |
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| source | Springer Link |
| subjects | Agriculture Applied Ecology Binding sites Biomedical and Life Sciences Cytochrome Cytochrome P450 Entomology Environmental Management Fenvalerate Helicoverpa armigera Insecticides Lepidoptera Life Sciences Noctuidae Open reading frames Original Research Paper Pesticide resistance Plant Pathology Transcription factors Zoology |
| title | Comparison of cytochrome P450 CYP332A1 gene in resistant and susceptible strains of the cotton bollworm, Helicoverpa armigera (Lepidoptera: Noctuidae) |
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