An injectable chitosan-based hydrogel reinforced by oxidized nanocrystalline cellulose and mineral trioxide aggregate designed for tooth engineering applications

The complex anatomy of teeth limits the accessibility and efficacy of regenerative treatments. Therefore, the application of well-known inducers as injectable hydrogels for the regeneration of the dentin-pulp complex is considered a promising approach. In this regard, this study aimed to develop an...

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Bibliographic Details
Published in:Cellulose (London) 2022-04, Vol.29 (6), p.3453-3465
Main Authors: Dalir Abdolahinia, Elaheh, Alipour, Mahdieh, Aghazadeh, Marziyeh, Hassanpour, Mehdi, Ghorbani, Marjan, Aghazadeh, Zahra
Format: Article
Language:English
Subjects:
Alizarin
Alkaline phosphatase
Bioorganic Chemistry
Cellulose
Ceramics
Chemistry
Chemistry and Materials Science
Chitosan
Composites
Dentin
Differentiation
Glass
Hydrogels
Nanocrystals
Natural Materials
Organic Chemistry
Original Research
Physical Chemistry
Physiochemistry
Polymer Sciences
Rheological properties
Scaffolds
Stem cells
Sustainable Development
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Summary:The complex anatomy of teeth limits the accessibility and efficacy of regenerative treatments. Therefore, the application of well-known inducers as injectable hydrogels for the regeneration of the dentin-pulp complex is considered a promising approach. In this regard, this study aimed to develop an injectable hydrogel containing mineral trioxide aggregate (MTA). The injectable chitosan/oxidized-nanocrystalline cellulose/MTA (CS/OCNC/MTA) hydrogels were prepared, and the physicochemical properties of these hydrogels were evaluated by TGA, FTIR, Rheological analysis, and SEM. Moreover, the effect of MTA on the swelling and degradability of scaffolds was assessed. The proliferative effects of synthesized hydrogels were also determined on human dental pulp stem cells (hDPSCs) by MTT assay. For induction of differentiation and biomineralization in these cells, the alkaline phosphatase activity and Alizarin Red S staining tests were performed in the presence of fabricated scaffolds. The proliferation of hDPSCs was significantly increased in the presence of these hydrogels. Moreover, the addition of MTA to hydrogel structure dramatically improved the differentiation of hDPSCs. These results suggested that this novel injectable hydrogel provides appropriate physiochemical properties and can be considered a promising scaffold for regenerative endodontic procedures. Graphical abstract
ISSN:0969-0239
1572-882X
DOI:10.1007/s10570-022-04491-z