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Application based Studies of HPTLC-bioautography in Evaluation of Botanicals: a Review

HPTLC is a widely used tool in standardization of herbs because of its ability to estimate the presence of active components in samples. HPTLC-bioautography is a technique for isolating active phytoconstituents on chromatograms based on their target. A large number of bioactive compounds with therap...

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Bibliographic Details
Published in:Journal of analytical chemistry (New York, N.Y.) N.Y.), 2022-04, Vol.77 (4), p.473-483
Main Authors: Santosh S. Bhujbal, Chawale, Bhushankumar G., Kale, Mayuri A.
Format: Article
Language:English
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Summary:HPTLC is a widely used tool in standardization of herbs because of its ability to estimate the presence of active components in samples. HPTLC-bioautography is a technique for isolating active phytoconstituents on chromatograms based on their target. A large number of bioactive compounds with therapeutic and nutritional relevance can be found in medicinal plants. It allows the quick, simple detection and isolation of bioactive compounds. It is a novel approach for evaluating these bioactive compounds having pharmacological effects. The technique is hyphenated with NMR/MS or Raman spectroscopy to determine the structural properties of compounds. This review summarizes and highlights the importance of this technique in today’s lifestyle, existing bioautographic technologies, and their application for detecting various pharmacological and other biological activities in plants. It also explains the current state and prospects of HPTLC-bioautography as well as its potential applications.
ISSN:1061-9348
1608-3199
DOI:10.1134/S1061934822040116