A Fluorescence Polarization Immunoassay Procedure for Determining Dibutyl Phthalate in Water

We developed a fluorescence polarization immunoassay (FPIA) procedure for determining the dibutyl phthalate (DBP) plasticizer, a toxic technogenic environmental pollutant, in water. The procedure is based on the competitive interaction of DBP and fluorophore-labeled DBP with antibodies in the sample...

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Bibliographic Details
Published in:Journal of analytical chemistry (New York, N.Y.) N.Y.), 2022-04, Vol.77 (4), p.466-472
Main Authors: Baranovskaya, V. S., Berlina, A. N., Eremin, S. A.
Format: Article
Language:English
Subjects:
Analysis
Analytical Chemistry
Antibodies
Chemistry
Chemistry and Materials Science
Dibutyl phthalate
Fluorescein
Fluorescence
Immunoassay
Polarization
Polarized light
Pollutants
Russia
Viral antibodies
Water pollution
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Summary:We developed a fluorescence polarization immunoassay (FPIA) procedure for determining the dibutyl phthalate (DBP) plasticizer, a toxic technogenic environmental pollutant, in water. The procedure is based on the competitive interaction of DBP and fluorophore-labeled DBP with antibodies in the sample, fluorescence excitation by plane-polarized light, and recording the fluorescence polarization of the emitted light. A conjugate of an amino derivative of fluorescein with succinate-dibutyl phthalate was synthesized, and its binding with polyclonal antibodies was confirmed. The FPIA procedure is characterized by a limit of detection for DBP of 0.35 µg/mL and an analytical range from 0.5 to 7.5 µg/mL. The analysis time is 2 min.
ISSN:1061-9348
1608-3199
DOI:10.1134/S1061934822040037