Anti-viral activity of methyl 1-chloro-7-methyl-2-propyl-1h-benzo[d] imidazole-5-carboxylate against white spot syndrome virus in freshwater crab (Paratelphusa hydrodromous)

Shrimp farming industries are subjected to severe economic loss due to a disease called white spot syndrome, a viral disease caused by white spot syndrome virus (WSSV) in penaeid shrimp. Numerous active compounds in the market possess anti-viral activity against the white spot syndrome virus, yet th...

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Bibliographic Details
Published in:Aquaculture international 2022-04, Vol.30 (2), p.989-998
Main Authors: Karthikeyan, K., Kumar, A. T. Manish, Harshini, D., Pooja, G., Daniel, J. Arul, Yasam, Pavani, Gracevictoria, G., Priayanka, M., Krishnaraj, P., Vidya, R., Devi, S. Asha, Reddy, Sabbasani Rajasekhara, Sudhakaran, Raja
Format: Article
Language:English
Subjects:
Aquaculture
Biomedical and Life Sciences
Body organs
Crustaceans
Decapoda
Economics
Freshwater
Freshwater & Marine Ecology
Freshwater crustaceans
Gills
Histopathology
Industry
Inland water environment
Life Sciences
Marine crustaceans
Muscles
Nucleotide sequence
PCR
Shellfish farming
Shrimp culture
Survival
Viral diseases
Viroses
White spot syndrome virus
Zoology
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Summary:Shrimp farming industries are subjected to severe economic loss due to a disease called white spot syndrome, a viral disease caused by white spot syndrome virus (WSSV) in penaeid shrimp. Numerous active compounds in the market possess anti-viral activity against the white spot syndrome virus, yet the issue remains unsolved. The present study was carried out to determine the anti-viral activity of methyl 1-chloro-7-methyl-2-propyl-1h-benzo[d] imidazole-5-carboxylate (C 13 H 15 N 2 O 2 Cl) against WSSV. The anti-viral activity of the synthetic compound was determined in freshwater crabs. Crabs were divided into three different experimental groups: healthy control groups (N.C.) received NTE buffer, positive control group (P.C.) crabs received WSSV, and treatment group crabs received WSSV along with synthetic weight compound. Experimental groups were observed for 30 days post-infection. Three different organs (gills, muscles, and head soft tissue (HST)) were dissected from all three groups and analyzed using molecular-based techniques, including polymerase chain reaction (PCR), Western blot, and histopathology. Clinical signs of WSSV were observed in the positive and N.C. groups; however, the treatment group showed a 100% survival rate. Confirmation was done using PCR, Western blot, and histopathology. These results demonstrated that the given synthetic compound has significant anti-viral activity against WSSV.
ISSN:0967-6120
1573-143X
DOI:10.1007/s10499-021-00714-5