In Situ Genetic Variability and Micropropagation of Cerastium banaticum (Rochel) Heuff. (Caryophyllaceae)– a Rare and Endemic Species from Romania

Rare and endemic plant species represent important components of plant biodiversity which require protection to ensure their sustainable conservation. Cerastium banaticum (Rochel) Heuff. is such an endemic and rare species from Romania, for which the genetic variability of two natural populations wa...

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Bibliographic Details
Published in:Acta biologica cracoviensia. Series Botanica. 2019-01, Vol.61 (1), p.53
Main Authors: Cristea, Victoria, Besenyei, Enikő, Jarda, Lilianna, Farkas, Anca, Marcu, Delia, Clapa, Doina, Halmagyi, Adela, Butiuc-Keul, Anca
Format: Article
Language:English
Subjects:
Acclimatization
Adenine
Biodiversity
Biology
Botanical gardens
Cerastium
Conservation
Culture media
Endemic plants
Endemic species
Flowers & plants
Genetic diversity
Genetic engineering
Genetic variability
Growth regulators
Micropropagation
Multiplication
Naphthaleneacetic acid
National parks
Natural populations
Plant growth
Plant species
Population
Population genetics
Population studies
Populations
Rare species
Rooting
Seeds
Shoots
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Summary:Rare and endemic plant species represent important components of plant biodiversity which require protection to ensure their sustainable conservation. Cerastium banaticum (Rochel) Heuff. is such an endemic and rare species from Romania, for which the genetic variability of two natural populations was studied by SSR markers. Shannon’s information index revealed low levels of genetic diversity in both populations (I = 0.296). As the first attempt in a conservation program a reproducible micropropagation protocol was established starting from seeds, followed by multiplication, rooting, and ex vitro acclimatization. Among the various plant growth regulators tested the highest multiplication coefficient was achieved on a culture medium with 0.5 mg L-1 6-furfurylaminopurine (K) and 1 mg L-1 α-naphthaleneacetic acid (NAA). On this PGRs concentration a number of 26.6 shoots/individual explant with a mean length of 7.9 cm for new generated shoots was registered. The highest number of roots/individual initiated shoot was 2.6 and it was recorded on a culture medium with 0.5 mg L-1 2-isopentyl-adenine (2iP) and 0.1 mg L-1 NAA. The outdoor acclimatization was successfully performed in a specially designed rocky area in the ‘Alexandru Borza’ Botanical Garden, Cluj-Napoca (Romania).
ISSN:0001-5296
1898-0295
DOI:10.24425/abcsb.2019.127737